The quest for reliable and versatile reagents is continuous in the scientific community. HEPES (4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid), identified by CAS 7365-45-9, is a widely adopted biological buffer known for its efficacy and broad applicability. As a dedicated manufacturer and supplier, we provide scientists with high-quality HEPES that fuels innovation across numerous research disciplines.

HEPES is a zwitterionic compound with a pKa of approximately 7.48 at 25°C, making it an excellent buffer for maintaining physiological pH conditions (6.8-8.2). This property is fundamental to its widespread use in cell biology. Researchers utilize HEPES in cell culture media to ensure consistent pH, which is critical for cell growth, viability, and the performance of various cellular processes. Its stability against fluctuating CO2 levels in incubators offers a significant advantage over bicarbonate-based buffers, leading to more predictable cell culture outcomes.

In the realm of enzymology, HEPES is equally indispensable. Enzymes are highly sensitive to pH, with their activity often peaking within a narrow range. HEPES provides the stable environment necessary for accurate enzyme kinetic studies, protein structure-function analyses, and enzyme assays used in drug discovery. Its minimal interaction with metal ions prevents unwanted chelation, ensuring that the enzyme's activity is not inadvertently affected by buffer components. For scientists looking to buy HEPES, our commitment to high purity (minimum 99.5%) ensures that your enzymatic experiments are not compromised by impurities.

The versatility of HEPES extends further into molecular biology, aiding in techniques such as PCR, DNA sequencing, and protein purification. Its consistent buffering capacity supports the optimal functioning of DNA polymerases and other enzymes critical for these processes. Moreover, HEPES can be incorporated into buffer formulations for chromatography, helping to maintain the integrity of protein samples during elution and separation.

As a leading supplier of research chemicals, we understand the importance of accessibility and affordability. Our expertise as a manufacturer in China allows us to offer competitive HEPES price points, making this essential reagent accessible to a broader range of research projects. We provide HEPES in a convenient free-flowing white powder form, packaged for optimal preservation of quality and ease of use in laboratory settings. We are committed to being a reliable partner, ensuring a consistent supply for your ongoing research needs.

For those seeking to procure HEPES (CAS 7365-45-9), we invite you to explore our product offerings. Our dedication to quality, combined with our robust supply chain and responsive customer service, makes us an ideal choice. Contact us today to request samples, obtain detailed product specifications, and secure a quotation for your next order of this versatile biological buffer. Let us help you achieve reliable and reproducible results in your diverse research endeavors.