The success of molecular biology and biochemical research often hinges on the precise control of experimental conditions, with pH stability being a critical factor. HEPES (4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid), designated by CAS 7365-45-9, is a paramount reagent that facilitates this control, making it indispensable in laboratories worldwide. As a premier manufacturer and supplier, we are dedicated to providing scientists with the high-quality HEPES necessary to optimize their research.

HEPES is celebrated for its robust buffering capabilities within the physiological pH range of 6.8 to 8.2, owing to its pKa of approximately 7.48 at 25°C. This characteristic is particularly vital in molecular biology applications where enzymes like polymerases, ligases, and nucleases operate optimally within narrow pH windows. Using HEPES in PCR buffer formulations, for instance, ensures consistent amplification of DNA, free from pH-induced variations that could lead to failed reactions or reduced yields. Similarly, in gel electrophoresis, HEPES-containing running buffers help maintain uniform electrical conductivity and pH across the gel, leading to sharper band separation and more accurate analysis of DNA or protein samples.

Beyond nucleic acid manipulation, HEPES plays a significant role in protein biochemistry. When researchers are studying enzyme kinetics, protein folding, or protein-ligand interactions, maintaining the correct pH is essential for preserving the protein's native structure and functional activity. HEPES buffers are favored for their chemical inertness, meaning they do not readily bind to proteins or metal ions, thus avoiding interference with the experimental system. For those looking to buy HEPES for these applications, sourcing from a reputable manufacturer like ourselves guarantees a high-purity product (minimum 99.5%) that minimizes potential assay interference.

The practical aspects of using HEPES are also noteworthy. Available typically as a free-flowing white powder, it is easy to measure and dissolve, a convenience that is highly valued in busy research environments. Our commitment as a supplier extends to providing this material in secure, appropriate packaging, ensuring its quality and stability during transit and storage. We understand the demands of research, and reliable access to quality reagents is paramount.

For procurement specialists and scientists in the field of molecular biology, identifying a dependable HEPES supplier is a strategic imperative. We offer competitive pricing and a consistent supply chain, making us an ideal partner for both small-scale research needs and larger bulk requirements. Our extensive experience as a manufacturer in China allows us to provide high-quality HEPES at attractive price points, supporting scientific innovation globally. We encourage you to reach out to us for detailed product information, sample requests, and a personalized quote.

In summary, HEPES (CAS 7365-45-9) is a critical reagent that significantly enhances the reliability and reproducibility of molecular biology and biochemical research. Its superior buffering capacity and chemical stability make it a preferred choice for a wide array of applications. Partner with us, a trusted manufacturer and supplier, to ensure you have access to the high-purity HEPES essential for your laboratory’s success. Contact us today to secure your supply.