Enzyme kinetics and activity assays are fundamental pillars of biochemical research, drug discovery, and diagnostic development. In this context, specific substrate molecules are employed to measure enzymatic function. 2-Nitrophenyl Beta-D-Glucopyranoside (CAS 2816-24-2) has emerged as a key player in this arena, particularly for enzymes that hydrolyze beta-glycosidic bonds.

What Makes it a Useful Substrate?
The utility of 2-Nitrophenyl Beta-D-Glucopyranoside stems from its chromogenic nature. When the beta-glycosidic bond linking the glucose unit to the 2-nitrophenyl group is cleaved by an appropriate enzyme, such as beta-glucosidase, it liberates 2-nitrophenol. This phenolic compound is a weak acid that, in a neutral or alkaline solution, exists in its deprotonated form, which is intensely yellow. The intensity of the yellow color produced is directly proportional to the amount of enzyme activity present, allowing for quantitative measurements using a spectrophotometer. This makes it an invaluable tool for researchers who need to buy a reliable substrate for their enzyme studies.

Applications in Research Settings
Researchers commonly purchase 2-Nitrophenyl Beta-D-Glucopyranoside for a variety of applications:

  • Enzyme Activity Assays: Measuring the specific activity of beta-glucosidase in biological samples, microbial cultures, or purified enzyme preparations.
  • Enzyme Inhibitor Screening: Evaluating the efficacy of potential drug candidates that inhibit beta-glucosidase activity.
  • Microbial Identification: Some diagnostic tests for identifying specific bacteria or fungi rely on their ability to produce enzymes that can hydrolyze substrates like this one.
  • Quality Control: Used by manufacturers to ensure the consistent activity of enzyme preparations or to test the quality of other glycoside-related products.

The consistent availability of high-purity 2-Nitrophenyl Beta-D-Glucopyranoside from reputable suppliers, including manufacturers in China, is vital for the reliability of these enzymatic studies. When you buy this product, you are investing in a proven tool that simplifies and quantifies complex enzymatic processes.