In the dynamic field of biochemical research, the selection of high-quality reagents is paramount to achieving accurate and reproducible results. Among these essential compounds, 2-Nitrophenyl Beta-D-Glucopyranoside stands out as a widely used and valuable tool. With its CAS number 2816-24-2, this glycoside plays a significant role in various enzymatic assays and studies.

Understanding 2-Nitrophenyl Beta-D-Glucopyranoside
This chemical compound, often abbreviated, is a derivative of glucose. Its molecular structure, C12H15NO8, with a molecular weight of 301.25, is specifically designed to act as a substrate for enzymes like beta-glucosidase. When acted upon by such enzymes, it undergoes hydrolysis, releasing p-nitrophenol. The p-nitrophenol then exhibits a distinct yellow color, making it easily detectable and quantifiable through spectrophotometry. This chromogenic property is the foundation of its utility in many scientific applications.

Key Applications in the Lab
The primary application of 2-Nitrophenyl Beta-D-Glucopyranoside is as a chromogenic substrate for the detection and quantification of beta-glucosidase activity. This enzyme is crucial in various biological processes, including the breakdown of complex carbohydrates and in microbial diagnostics. Researchers often buy this reagent to assess enzyme kinetics, screen for enzyme inhibitors, or determine the presence and activity of specific microorganisms in samples.

Beyond enzyme assays, it can also serve as an intermediate in organic synthesis, particularly in the development of more complex glycosylated molecules or pharmaceutical compounds. For researchers in life sciences, sourcing this chemical from a reputable manufacturer or supplier is crucial. Ensuring consistent purity and reliable availability from a trusted source, such as a manufacturer in China, can significantly impact the success of experimental protocols. When looking to buy this product, consider suppliers who provide detailed specifications and certificates of analysis to guarantee quality for your critical research needs.