The field of molecular biology and genetic research heavily relies on the ability to synthesize specific DNA and RNA sequences. This process, known as oligonucleotide synthesis, requires a suite of specialized chemical reagents, with phosphoramidites being among the most critical. For researchers and procurement managers, understanding these reagents and how to source them effectively is key to successful outcomes. This guide focuses on the acquisition of 2-Cyanoethyl N,N,N',N'-tetraisopropylphosphorodiamidite (CAS: 102691-36-1), a pivotal compound in this domain.

At NINGBO INNO PHARMCHEM CO.,LTD., we are dedicated to providing the scientific community with high-quality chemical building blocks. Our expertise as a manufacturer and supplier of specialty chemicals means we understand the precise needs of our clients in oligonucleotide synthesis.

The Indispensable Role of Phosphoramidites

Phosphoramidites are the workhorses of modern automated oligonucleotide synthesis. They are protected nucleoside derivatives that readily react with a growing oligonucleotide chain attached to a solid support. The 2-cyanoethyl group attached to the phosphorus atom in 2-Cyanoethyl N,N,N',N'-tetraisopropylphosphorodiamidite serves as a crucial protecting group, which is later removed under specific conditions. The tetraisopropyl groups contribute to the reagent's stability and reactivity profile.

When you need to buy reagents for DNA or RNA synthesis, the choice of phosphoramidite significantly impacts the efficiency and purity of the final product. 2-Cyanoethyl N,N,N',N'-tetraisopropylphosphorodiamidite is favored for its:

  • High Coupling Efficiency: It reacts swiftly and effectively with deprotected nucleosides, leading to longer and more accurate oligonucleotide chains.
  • Stability: It offers better stability compared to some older phosphitylating agents, simplifying storage and handling.
  • Cost-Effectiveness: It provides a balance of performance and economic viability for both research and large-scale production.

Typically supplied as a colorless, transparent liquid with a purity often exceeding 95%, this compound is essential for applications ranging from academic research to the production of therapeutic oligonucleotides.

Navigating Your Purchase: What to Look for in a Supplier

For procurement managers and researchers, selecting the right supplier for critical reagents like phosphoramidites is a strategic decision. Here are key factors to consider when you decide to buy:

  • Manufacturer Reputation: Partner with established manufacturers known for their quality control and reliability.
  • Product Specifications: Ensure the product meets the required purity (e.g., ≥95%) and has consistent lot-to-lot quality.
  • Supply Chain Security: A supplier's ability to maintain consistent stock and provide timely delivery is crucial for ongoing projects.
  • Technical Support: Access to knowledgeable technical support can resolve application-specific queries and troubleshooting.
  • Competitive Pricing: While quality is paramount, competitive pricing ensures cost-efficiency for your synthesis endeavors.

As a leading manufacturer and supplier of high-quality chemical intermediates, we at NINGBO INNO PHARMCHEM CO.,LTD. are committed to meeting these needs. We offer 2-Cyanoethyl N,N,N',N'-tetraisopropylphosphorodiamidite with guaranteed purity and backed by reliable customer service. We aim to be your trusted partner in securing the essential reagents that drive scientific progress in oligonucleotide synthesis.