For researchers in life sciences, bioconjugation is a cornerstone technique that enables a vast array of experimental and diagnostic applications. At the heart of many biotinylation strategies lies reagents like Sulfo-NHS-LC-Biotin. As a premier manufacturer and supplier of high-quality chemical reagents, we understand the critical importance of precise and reliable tools for your work. This article delves into the utility of Sulfo-NHS-LC-Biotin (CAS 191671-46-2), a vital component for scientists seeking to label biomolecules.

Sulfo-NHS-LC-Biotin is specifically designed for amine-reactive labeling. Its structure features a succinimidyl ester group that reacts readily with primary amines (like lysine residues on proteins) under mild conditions. The 'LC' in its name signifies a 'long-chain' spacer, which can be beneficial in reducing steric hindrance between the biotin and the conjugated molecule, thereby preserving the binding affinity of both. The 'Sulfo' prefix indicates its water solubility, a significant advantage over its non-sulfonated counterparts, making it ideal for reactions in aqueous buffers, which is often the preferred environment for biological molecules.

The primary application of Sulfo-NHS-LC-Biotin is the covalent attachment of biotin to proteins, peptides, antibodies, and other amine-containing biomolecules. Once biotinylated, these molecules can be readily detected or purified using avidin or streptavidin conjugates, which have an exceptionally high binding affinity for biotin. This avidin-biotin system is fundamental in numerous laboratory techniques, including:

  • ELISA (Enzyme-Linked Immunosorbent Assay): Biotinylated antibodies can be detected with enzyme-conjugated avidin, amplifying the signal for more sensitive detection.
  • Western Blotting: Similarly, biotinylated detection antibodies or probes allow for enhanced visualization of target proteins on membranes.
  • Immunohistochemistry (IHC) and Immunocytochemistry (ICC): Biotinylated antibodies improve the signal-to-noise ratio in staining tissues and cells.
  • Affinity Chromatography: Biotinylated targets can be captured on avidin- or streptavidin-coated matrices for purification.
  • Flow Cytometry: Biotinylated antibodies or probes can be used for cell surface marker detection or intracellular staining.

When considering where to buy Sulfo-NHS-LC-Biotin, it's crucial to prioritize purity and consistency. Our company, a reputable Sulfo-NHS-LC-Biotin supplier and manufacturer in China, guarantees a high purity of ≥99% for this reagent. This level of purity is essential to minimize side reactions and ensure the specificity and reliability of your experiments. We understand that reliable sourcing is key for R&D scientists and procurement managers, which is why we focus on providing consistent quality and competitive Sulfo-NHS-LC-Biotin price points.

The storage conditions for Sulfo-NHS-LC-Biotin are typically at -20°C to maintain its stability. Its water solubility simplifies reaction setup, as it can be dissolved in water or other aqueous buffers to the desired concentration. For researchers looking to optimize their bioconjugation strategies and secure a dependable supply of this vital chemical, reaching out to a trusted CAS 191671-46-2 manufacturer is the most strategic approach. We are committed to supporting your scientific endeavors by providing the high-quality reagents you need.