Biotinylation, the process of covalently attaching biotin to a molecule, is a foundational technique in molecular biology and biotechnology. It leverages the exceptionally strong non-covalent interaction between biotin and avidin/streptavidin to enable sensitive detection, purification, and immobilization of biomolecules. At the forefront of this technique is the use of specific biotinylation reagents, and Sulfo-NHS-LC-Biotin (CAS 191671-46-2) is a prime example of a highly effective and widely utilized compound. As a dedicated Sulfo-NHS-LC-Biotin manufacturer and supplier, we aim to provide researchers with the tools they need to succeed.

Sulfo-NHS-LC-Biotin is an amine-reactive reagent, meaning it targets the primary amine groups (typically on lysine residues or N-termini) present in proteins and other biomolecules. The reaction proceeds via nucleophilic attack by the amine on the N-hydroxysuccinimide (NHS) ester, forming a stable amide bond. The presence of the sulfonate group confers significant water solubility, allowing for efficient reactions in aqueous buffers without the need for organic co-solvents, which can sometimes denature sensitive biological samples. The long chain (LC) linker provides a degree of spatial separation between the biotin and the conjugated molecule, which can be advantageous for optimal avidin/streptavidin binding and reduced steric hindrance. When scientists need to buy Sulfo-NHS-LC-Biotin, they often look for these specific properties.

The practical utility of Sulfo-NHS-LC-Biotin spans a broad spectrum of research and diagnostic applications:

  • Antibody Labeling for Immunoassays: Biotinylated antibodies are crucial for highly sensitive ELISA, Western Blots, and IHC/ICC, enabling robust detection.
  • Protein Detection and Purification: Labeling proteins allows for their capture on avidin-coated surfaces or beads for purification or detection in complex mixtures.
  • Nucleic Acid Labeling: While less common, it can be adapted for labeling amine-modified oligonucleotides.
  • Drug Delivery Systems: Biotinylation can be used to attach targeting moieties to drug carriers or nanoparticles.
  • Diagnostic Kit Components: It serves as a key component in the development of sensitive diagnostic assays.

For researchers and procurement specialists, securing a high-quality, reliable source for this reagent is paramount. Our position as a leading Sulfo-NHS-LC-Biotin supplier and manufacturer in China ensures that we can offer products with ≥99% purity, critical for demanding scientific applications. We are committed to providing competitive Sulfo-NHS-LC-Biotin price options, making advanced research accessible. Sourcing directly from a reputable CAS 191671-46-2 supplier like us simplifies procurement and assures quality.

To maintain its efficacy, Sulfo-NHS-LC-Biotin should be stored at -20°C. Its water solubility is a significant practical advantage, allowing for easy preparation of reaction solutions. Whether you are developing cutting-edge research tools or routine diagnostic assays, the quality of your reagents directly impacts your results. We are dedicated to providing the high-quality chemicals that empower scientific innovation.