In the intricate world of protein chemistry, maintaining the correct structure and function is paramount. A common challenge faced by researchers and manufacturers is the unintended formation of disulfide bonds between cysteine residues. These bonds, while sometimes functional, can also lead to misfolding, aggregation, or loss of biological activity. This is where Dithiothreitol (DTT), also known as Cleland's reagent, plays a pivotal role.

DTT is a powerful reducing agent widely recognized for its ability to cleave disulfide bonds. Its unique chemical structure, featuring two thiol (-SH) groups, allows it to readily donate electrons, thereby reducing disulfide linkages (-S-S-) back to their free thiol forms. This process is essential for unfolding proteins, reducing protein aggregation, and preparing proteins for downstream analyses such as SDS-PAGE or mass spectrometry. As a leading DTT manufacturer and supplier, we understand the critical need for high-purity reagents in these sensitive applications.

The effectiveness of DTT is particularly pronounced in aqueous solutions and at pH values above 7, where its thiol groups are deprotonated, increasing their reactivity. This pH dependency is a key factor to consider when designing experimental protocols. When you buy DTT from us, you are assured of a product with an assay typically exceeding 98%, minimizing impurities that could interfere with your protein reduction processes. We are committed to providing reliable DTT for purchase to support your scientific endeavors.

Beyond breaking existing disulfide bonds, DTT also acts as an antioxidant, preventing the re-oxidation of free thiols. This protective function is crucial during protein purification and storage, helping to maintain the integrity of proteins that rely on free sulfhydryl groups for their activity. For those seeking a consistent and high-quality source, our DTT offerings as a China-based supplier are designed to meet the demands of both laboratory research and larger-scale biopharmaceutical production. Explore how our DTT can enhance your protein purification strategies and ensure stable, functional protein products.