Förster Resonance Energy Transfer (FRET) is a powerful biophysical technique that allows scientists to study molecular interactions and conformational changes in real-time. At the heart of FRET assays are donor and acceptor fluorophores, and the judicious selection of these components is critical for assay sensitivity and specificity. Among the widely used acceptor fluorophores, 6-Carboxytetramethylrhodamine (CAS 91809-67-5) stands out for its excellent spectral characteristics and conjugation capabilities. For researchers and procurement specialists, understanding its role and how to source it effectively is key.

6-Carboxytetramethylrhodamine, a rhodamine derivative, exhibits strong absorption in the visible spectrum and emits fluorescence in the orange-red region. Its emission spectrum is well-suited for pairing with a variety of donor fluorophores in FRET experiments. The efficiency of FRET is highly dependent on the spectral overlap between the donor's emission and the acceptor's excitation, as well as the distance between the two molecules. The consistent spectral properties of high-purity 6-Carboxytetramethylrhodamine make it a reliable choice for developing robust FRET-based assays.

As a dedicated manufacturer and supplier, we provide 6-Carboxytetramethylrhodamine with meticulous quality control to ensure optimal performance in sensitive applications like FRET. Our product is available with high assay purity (e.g., ≥95%), which is crucial because impurities can interfere with FRET efficiency and lead to misleading results. When you are looking to buy this vital component, consider partnering with a supplier in China that emphasizes consistency and offers competitive pricing, ensuring your research budget is well-managed.

The conjugation of 6-Carboxytetramethylrhodamine to biomolecules, such as peptides or proteins, is typically achieved through its carboxylic acid functionality. This often involves activation with reagents like succinimidyl esters or carbodiimides to form stable amide bonds with amine groups on the target molecule. The success of the FRET assay relies not only on the dye's spectral properties but also on the efficiency and specificity of this conjugation process. Therefore, sourcing dyes from a reputable manufacturer who understands these biochemical processes is advantageous.

For procurement managers and research scientists, securing a dependable supply chain for critical reagents like CAS 91809-67-5 is essential for uninterrupted research. We aim to be that reliable partner, offering not just high-quality products but also responsive customer service and timely delivery. Whether you are developing novel biosensors, studying protein-protein interactions, or quantifying enzyme activity, our 6-Carboxytetramethylrhodamine can be a valuable asset to your laboratory.

In conclusion, the application of 6-Carboxytetramethylrhodamine in FRET assays highlights its significance as a fluorescent labeling tool. By choosing a trusted supplier for this essential reagent, you ensure the accuracy and sensitivity required for cutting-edge molecular research. We encourage you to contact us to learn more about our offerings and how we can support your FRET assay development needs.