Molecular biology relies heavily on precise control of reaction conditions, and the role of metal ions, particularly calcium, is often central to these processes. Ethylenebis(oxyethylenenitrilo)tetraacetic Acid (EGTA, CAS 67-42-5) is a critical reagent for researchers in this field due to its exceptional ability to chelate calcium ions selectively. As a prominent manufacturer and supplier of biochemicals in China, we are dedicated to providing researchers with the high-purity reagents they need.

EGTA is characterized by its white crystalline powder form and its solubility in alcohol-based solvents, making it easy to incorporate into aqueous buffer systems commonly used in molecular biology. Its chemical structure, C14H24N2O10, allows it to form stable complexes with calcium ions, effectively lowering the concentration of free Ca2+ in solution. This selective calcium binding is its most significant attribute, differentiating it from more general chelators like EDTA.

One of the primary applications of EGTA in molecular biology is as a buffer component. Many enzymes and cellular processes are highly sensitive to calcium levels. For instance, in studies involving calcium-dependent enzymes or proteins that interact with calcium, EGTA is used to maintain a low, controlled concentration of free calcium. This ensures that the observed activity or interaction is truly mediated by the intended calcium levels and not by ambient calcium present in reagents or water.

Furthermore, EGTA is invaluable in enzyme assays where calcium might interfere with the reaction or affect enzyme stability. By adding EGTA, researchers can effectively 'turn off' calcium-dependent reactions or precisely titrate calcium to study enzyme kinetics. For those looking to buy EGTA for such purposes, ensuring a high purity level, such as our offered 99% minimum assay, is paramount for experimental reliability.

Sample preparation in molecular biology can also benefit from EGTA. It can be used to stabilize biological samples by chelating calcium that might activate certain degradation pathways. For laboratories requiring a consistent and quality supply of EGTA, partnering with a reputable manufacturer in China offers significant advantages, including competitive pricing and a robust supply chain. We are committed to supporting the advancements in molecular biology by providing essential tools like EGTA. Contact us to discuss your requirements and purchase EGTA for your critical research applications.