In the intricate world of biological and biochemical research, maintaining a stable pH environment is paramount for the success of experiments. Among the array of buffering agents available, HEPPS (4-(2-Hydroxyethyl)piperazine-1-propanesulfonic acid), with CAS number 16052-06-5, stands out as a highly effective and widely utilized zwitterionic buffer. As a key supplier of this critical reagent, we understand its significance in driving scientific advancement.

HEPPS is prized for its ability to maintain a physiological pH range, typically between 7.3 and 8.7, with a pKa of approximately 8.0 at 25°C. This characteristic makes it an excellent choice for applications where cells or biomolecules must be kept within their optimal pH tolerance. Unlike some other buffers, HEPPS exhibits low metal ion chelation capabilities and minimal absorption in UV and visible light regions, further enhancing its suitability for sensitive assays.

One of the primary applications of HEPPS is in cell culture media. Maintaining a stable pH is crucial for cell viability, growth, and metabolic function. By using HEPPS, researchers can ensure that their cell cultures are provided with a consistent and supportive environment, leading to more reliable experimental outcomes. Whether you are conducting research in biopharmaceuticals, regenerative medicine, or basic cell biology, the consistent quality of HEPPS from a reliable manufacturer is essential.

Beyond cell culture, HEPPS plays a vital role in biochemical assays. Many enzymatic reactions and protein-binding studies are highly pH-dependent. The precise buffering capacity of HEPPS allows scientists to fine-tune experimental conditions, ensuring accurate measurement of enzyme activity, protein interactions, and other biochemical processes. Researchers looking to buy HEPPS for these demanding applications can rely on its consistent performance.

In the realm of molecular biology, HEPPS also finds significant utility. It is often employed in solutions for PCR (Polymerase Chain Reaction), DNA/RNA purification, and protein analysis techniques like SDS-PAGE. Its compatibility with various biological molecules and its ability to provide a stable ionic environment contribute to the efficiency and accuracy of these processes. For labs needing to purchase this versatile reagent, sourcing from a reputable supplier in China ensures both quality and cost-effectiveness.

The chemical structure of HEPPS, with its piperazine ring and sulfonic acid group, contributes to its zwitterionic nature, granting it high water solubility and minimal cell membrane permeability. These properties are fundamental to its effectiveness as a buffer in aqueous biological systems. As a manufacturer committed to providing high-grade chemicals, we ensure that our HEPPS product meets stringent purity standards, typically exceeding 99% assay.

For scientists and procurement managers seeking to purchase HEPPS, understanding its applications and the importance of purity is key. Whether your work involves advanced protein research, delicate assay development, or large-scale bioprocessing, investing in high-quality HEPPS is an investment in the integrity of your research. We encourage you to request a quote for our competitively priced HEPPS, available from our manufacturing facility.