In the dynamic world of biological research and pharmaceutical development, accurately assessing cell viability is paramount. Researchers constantly seek reliable methods to evaluate the health and functional status of cells, especially when exposed to various compounds or environmental stresses. One of the most established and widely utilized techniques for this purpose is the Neutral Red uptake assay. As a dedicated manufacturer and supplier of high-quality research chemicals, we understand the critical importance of this reagent.

Neutral Red, identified by CAS number 553-24-2, is a cationic vital stain renowned for its ability to accumulate within the lysosomes of living cells. This property makes it an excellent indicator of cellular integrity and metabolic activity. When introduced to a cell culture, live cells actively transport the dye into their lysosomes, which are acidic intracellular compartments. The accumulation of Neutral Red within these organelles provides a quantifiable measure of cell viability. Conversely, cells that are damaged or undergoing apoptosis typically exhibit reduced uptake or retention of the dye, signaling compromised viability.

The process is straightforward yet powerful. After exposing cells to the compound or treatment of interest, Neutral Red solution is added to the culture medium. Following a suitable incubation period, the excess dye is washed away, and the internalized dye is extracted from the cells, often using an acidified ethanol solution. The released dye is then quantified spectrophotometrically, typically at an absorbance maximum around 530 nm. The intensity of the color directly correlates with the number of viable cells present in the culture.

For researchers looking to buy Neutral Red dye online, it is crucial to source from a reputable supplier that guarantees purity and consistency. Our commitment as a leading manufacturer in China ensures that you receive a product meeting stringent quality standards, essential for obtaining reproducible and reliable results in your cell viability assays. Whether you are conducting drug screening, toxicology studies, or basic cell biology research, understanding how to effectively buy Neutral Red is key to successful experimentation.

Beyond viability assays, Neutral Red also finds application as a histological stain, highlighting lysosomes and other acidic structures within tissues. Its versatility extends to a role as a pH indicator, changing from red in acidic conditions to yellow in alkaline environments, a property useful in various biochemical applications. For those needing a reliable source for Neutral Red CAS 553-24-2, we provide comprehensive product information and support, ensuring you can make an informed purchase. Invest in quality and ensure the accuracy of your research by choosing a trusted manufacturer for your critical reagents.