Chemiluminescence detection has revolutionized biochemical and molecular biology research, offering unparalleled sensitivity and a broad dynamic range for detecting target molecules. At the heart of many chemiluminescent assays, particularly those involving alkaline phosphatase (AP) conjugates, lies a critical substrate: 5-Bromo-4-chloro-3-indolyl Phosphate Disodium Salt, or BCIP (CAS 102185-33-1). As a specialized chemical manufacturer, we understand the nuances of using such advanced reagents and are committed to supplying products that elevate your research capabilities.

BCIP, when used in conjunction with other reagents like Nitroblue tetrazolium (NBT), generates a highly sensitive chromogenic and chemiluminescent signal upon enzymatic hydrolysis by alkaline phosphatase. This characteristic makes it an invaluable tool for researchers looking to buy BCIP for applications requiring low-detectable limits.

Enhancing Sensitivity in Detection

The power of BCIP in chemiluminescence stems from its ability to produce a stable, intense signal. When AP cleaves the phosphate group from BCIP, it releases an indolyl derivative that can then participate in further reactions, often leading to the formation of brightly luminescent compounds. This process is crucial for detecting minute quantities of target analytes, such as specific proteins or nucleic acid sequences in blotting or immunoassay formats. A reliable 5-bromo-4-chloro-3-indolyl phosphate disodium salt supplier ensures that the substrate's quality directly translates into the sensitivity and reliability of your chemiluminescence results.

Applications Driving Innovation

Our high-purity BCIP is a key component in:

  • Western Blotting: Detecting target proteins conjugated with AP-labeled antibodies with superior sensitivity.
  • ELISA (Enzyme-Linked Immunosorbent Assay): Enabling highly sensitive detection of antigens or antibodies in complex biological samples.
  • Immunohistochemistry (IHC) and Immunocytochemistry (ICC): Visualizing AP activity in cellular and tissue samples, often yielding crisp signals for localization studies.
  • Nucleic Acid Detection: Used in hybridization assays where AP-labeled probes are employed for detecting specific DNA or RNA sequences.

Partnering for Research Success

As a dedicated supplier of biochemical reagents, we are focused on providing researchers with the tools they need to push the boundaries of scientific discovery. If you are seeking to enhance your chemiluminescence assays or require a dependable source for CAS 102185-33-1, consider our commitment to quality and service. We ensure that when you purchase BCIP from us, you are investing in precision and reliability for your advanced research needs.