Histochemistry, the study of the distribution of chemical components in biological tissues, relies heavily on precise visualization techniques. For researchers studying alkaline phosphatase (AP) activity, a crucial enzyme involved in various cellular processes, 5-Bromo-4-chloro-3-indolyl Phosphate Disodium Salt (BCIP, CAS 102185-33-1) is an indispensable reagent. As a specialized chemical manufacturer and supplier, we provide high-quality BCIP that enables clear and accurate visualization of enzyme localization within tissues.

BCIP acts as a chromogenic substrate. When hydrolyzed by alkaline phosphatase, it generates an insoluble blue precipitate at the site of enzyme activity. This precipitation allows histologists and researchers to pinpoint the exact location of AP within cells and tissue structures, providing invaluable insights into cellular function, disease pathology, and developmental biology. For those looking to buy BCIP for their histochemical needs, selecting a reliable supplier is critical for obtaining consistent, vibrant staining.

Achieving Clear Staining with BCIP

The effectiveness of BCIP in histochemistry depends on several factors, including its purity and the precise formulation of the staining solution. When used in combination with substrates like Nitroblue Tetrazolium (NBT), BCIP produces a distinct blue color (or a purplish-blue precipitate with NBT), which is easily discernible under a microscope. This combination is widely adopted for its sensitivity and the quality of the resultant precipitate, which tends to remain localized, preventing diffusion and ensuring sharp contrast.

We, as a dedicated 5-bromo-4-chloro-3-indolyl phosphate disodium salt supplier, ensure our product’s consistent quality, which translates to:

  • Sharp Localization: The insoluble precipitate formed by BCIP hydrolysis ensures that the blue color remains precisely at the site of alkaline phosphatase activity.
  • High Contrast: The distinct blue color provides excellent contrast against tissue backgrounds, facilitating clear observation and interpretation.
  • Reproducible Results: Our stringent quality control processes mean you can consistently achieve reliable staining patterns across multiple experiments.

Applications in Histological Research

Our BCIP finds extensive use in:

  • Tissue Staining: Visualizing the distribution of alkaline phosphatase in biopsy samples for diagnostic purposes or research.
  • Cellular Localization Studies: Identifying cellular compartments or specific cell types that express alkaline phosphatase.
  • Developmental Biology: Tracking gene expression patterns where AP reporter genes are utilized.

For researchers needing to purchase BCIP or inquiring about the CAS 102185-33-1 price, we offer a dependable solution. Partner with us, a trusted biochemical reagent supplier, to ensure the clarity and accuracy of your histochemical analyses.