In the realm of biochemical detection, few substrates are as versatile and widely recognized as 5-Bromo-4-chloro-3-indolyl Phosphate Disodium Salt, known by its acronym BCIP (CAS 102185-33-1). As a premier chemical manufacturer specializing in fine chemicals, understanding the science behind our products is key to appreciating their value. BCIP is a chromogenic substrate designed specifically for the enzymatic detection of alkaline phosphatase (AP), a critical enzyme in numerous biological processes and laboratory techniques.

The molecular structure of BCIP features an indole ring substituted with bromine and chlorine atoms, attached to a phosphate group. This structure is key to its function. When alkaline phosphatase acts upon BCIP, it hydrolyzes the phosphate ester bond. This enzymatic reaction liberates 5-bromo-4-chloro-3-hydroxyindole. This intermediate compound is unstable and readily undergoes oxidation and dimerization, typically in the presence of an oxidizing agent and often in conjunction with a coupling agent like Nitroblue Tetrazolium (NBT). The resulting product is an intensely colored, insoluble precipitate – usually blue or purplish-blue – which allows for clear visual detection and localization of AP activity.

The Mechanism of BCIP Action:

  1. Enzymatic Hydrolysis: Alkaline phosphatase catalyzes the removal of the phosphate group from BCIP.
  2. Indole Intermediate Formation: This releases 5-bromo-4-chloro-3-hydroxyindole.
  3. Oxidation and Dimerization: The intermediate is oxidized, forming an intensely colored dimer.
  4. Precipitation: The colored product is insoluble, precipitating at the site of enzyme activity.

Significance for Researchers and Industry

The ability of BCIP to produce a distinct, localized color change makes it invaluable for applications such as Western blotting, ELISA, immunohistochemistry, and in situ hybridization. Its sensitivity and the visual clarity of the reaction are why researchers consistently seek to buy BCIP from reliable sources. As a dedicated 5-bromo-4-chloro-3-indolyl phosphate disodium salt supplier, we ensure that our BCIP is produced under strict quality controls to deliver optimal performance. This guarantees that when you purchase BCIP, you are obtaining a reagent that enables precise and reproducible results.

For those in need of high-quality CAS 102185-33-1, whether for laboratory research or industrial applications, we stand ready as your trusted chemical intermediate supplier. We offer competitive pricing and consistent product availability, ensuring your research or production needs are met efficiently and effectively.