Understanding EGS: A Key Reagent for Protein Crosslinking and Stabilization
In the realm of protein science, controlling the structure and function of proteins is paramount for unlocking their potential in research, diagnostics, and therapeutics. Ethylene Glycol-bis(succinic acid N-hydroxysuccinimide ester) (EGS) is a widely adopted homobifunctional crosslinker that proves invaluable for protein crosslinking and stabilization. Ningbo Inno Pharmchem Co., Ltd. is a premier supplier of high-quality EGS, supporting scientists worldwide in their protein-related research. Learning about EGS and deciding to purchase it can significantly enhance experimental outcomes.
EGS is characterized by its two reactive N-hydroxysuccinimide (NHS) ester groups, which readily react with primary amine groups present in proteins, particularly the epsilon-amino groups of lysine residues and the alpha-amino group at the N-terminus. This reaction forms a stable amide bond, effectively crosslinking two protein molecules or different parts of the same protein. The ethylene glycol linker provides a 12-atom spacer, which is important for preventing steric hindrance and allowing the crosslinked protein to maintain its native conformation or function. When researchers seek to buy reagents for precise protein modification, EGS is often a top choice.
One of the primary uses of EGS is in protein stabilization. By introducing crosslinks, researchers can increase the rigidity of protein structures, thereby enhancing their resistance to denaturation by heat, pH changes, or proteolysis. This stabilization is particularly beneficial when working with fragile proteins or when proteins need to withstand harsh experimental conditions. For example, stabilized enzymes might be used in industrial applications or in diagnostic kits that require long shelf life.
EGS also plays a crucial role in various protein labeling and conjugation strategies. It can be used to attach labels such as fluorescent dyes, biotin, or enzymes to proteins, facilitating their detection and quantification in various biological assays. Moreover, EGS is a key component in the synthesis of antibody-drug conjugates (ADCs), where it acts as a linker to connect a cytotoxic drug to an antibody for targeted cancer therapy. The decision to purchase EGS for these applications is driven by its proven efficacy and the cleavable nature of its linker arm, which allows for controlled drug release.
Ningbo Inno Pharmchem Co., Ltd. is dedicated to providing the scientific community with reliable and high-purity chemical reagents. Our EGS is manufactured under stringent quality control protocols to ensure consistent performance. We understand the critical nature of protein crosslinking and stabilization in modern biological research and are committed to being a dependable supplier. Partner with Ningbo Inno Pharmchem Co., Ltd. for your EGS needs and advance your protein science research.
EGS is characterized by its two reactive N-hydroxysuccinimide (NHS) ester groups, which readily react with primary amine groups present in proteins, particularly the epsilon-amino groups of lysine residues and the alpha-amino group at the N-terminus. This reaction forms a stable amide bond, effectively crosslinking two protein molecules or different parts of the same protein. The ethylene glycol linker provides a 12-atom spacer, which is important for preventing steric hindrance and allowing the crosslinked protein to maintain its native conformation or function. When researchers seek to buy reagents for precise protein modification, EGS is often a top choice.
One of the primary uses of EGS is in protein stabilization. By introducing crosslinks, researchers can increase the rigidity of protein structures, thereby enhancing their resistance to denaturation by heat, pH changes, or proteolysis. This stabilization is particularly beneficial when working with fragile proteins or when proteins need to withstand harsh experimental conditions. For example, stabilized enzymes might be used in industrial applications or in diagnostic kits that require long shelf life.
EGS also plays a crucial role in various protein labeling and conjugation strategies. It can be used to attach labels such as fluorescent dyes, biotin, or enzymes to proteins, facilitating their detection and quantification in various biological assays. Moreover, EGS is a key component in the synthesis of antibody-drug conjugates (ADCs), where it acts as a linker to connect a cytotoxic drug to an antibody for targeted cancer therapy. The decision to purchase EGS for these applications is driven by its proven efficacy and the cleavable nature of its linker arm, which allows for controlled drug release.
Ningbo Inno Pharmchem Co., Ltd. is dedicated to providing the scientific community with reliable and high-purity chemical reagents. Our EGS is manufactured under stringent quality control protocols to ensure consistent performance. We understand the critical nature of protein crosslinking and stabilization in modern biological research and are committed to being a dependable supplier. Partner with Ningbo Inno Pharmchem Co., Ltd. for your EGS needs and advance your protein science research.
Perspectives & Insights
Alpha Spark Labs
“The decision to purchase EGS for these applications is driven by its proven efficacy and the cleavable nature of its linker arm, which allows for controlled drug release.”
Future Pioneer 88
“is dedicated to providing the scientific community with reliable and high-purity chemical reagents.”
Core Explorer Pro
“Our EGS is manufactured under stringent quality control protocols to ensure consistent performance.”