Histochemistry, the study of the microscopic structure of tissues and organs based on their chemical composition, often relies on specific staining techniques to reveal cellular components and enzymatic activities. NINGBO INNO PHARMCHEM CO.,LTD. is proud to supply 5-bromo-4-chloro-3-indolyl β-D (CAS: 114162-64-0), a versatile chromogenic substrate that significantly enhances histochemical staining protocols.

The primary advantage of 5-bromo-4-chloro-3-indolyl β-D in histochemistry lies in its ability to produce a visible color change upon enzymatic reaction. Specifically, it is used in conjunction with enzymes like β-glucuronidase (GUS). When applied to tissue samples containing this enzyme, the substrate is hydrolyzed, yielding an insoluble blue precipitate at the site of enzymatic activity. This localized color deposition allows researchers to pinpoint the exact location of the enzyme within the tissue, providing critical insights into cellular function and distribution. This precision is invaluable when studying gene expression patterns or the localization of specific cellular processes.

For researchers investigating Gus gene expression in tissues, this substrate serves as an excellent reporter system. By targeting tissues where the Gus gene is known or suspected to be active, scientists can visualize the spatial and temporal patterns of gene expression. This is particularly relevant in developmental biology and disease research, where understanding the precise localization of gene products is key to deciphering biological mechanisms. Our role as a reliable supplier means that you can consistently obtain high-purity 5-bromo-4-chloro-3-indolyl β-D for your demanding histochemical applications. NINGBO INNO PHARMCHEM CO.,LTD. is committed to supporting your research with quality biochemicals.

The application of 5-bromo-4-chloro-3-indolyl β-D is not limited to enzyme detection; it is also instrumental in diagnostic assays where the presence of specific enzymatic markers indicates certain cellular states or conditions. Its reliable performance in generating a clear, localized color signal makes it a preferred choice for many histochemical staining procedures. By choosing NINGBO INNO PHARMCHEM CO.,LTD., you are partnering with a dedicated manufacturer and supplier committed to delivering the highest quality products for your scientific endeavors, ensuring that your histochemical analyses are both precise and informative.