Fmoc-O-tert-butyl-L-serine: A Cornerstone of Modern Peptide Synthesis
In the dynamic field of peptide synthesis, the choice of building blocks significantly dictates the success of a project. Among these crucial components, Fmoc-O-tert-butyl-L-serine stands out as an indispensable reagent. As a leading manufacturer in China, NINGBO INNO PHARMCHEM CO.,LTD. is proud to supply this high-purity amino acid derivative that empowers researchers in drug development and beyond.
Fmoc-O-tert-butyl-L-serine, also known by its CAS number 71989-33-8, is a derivative of the amino acid L-serine. Its defining features are the Fmoc (9-fluorenylmethyloxycarbonyl) protecting group attached to the alpha-amino group and a tert-butyl ether protecting group on the side chain hydroxyl. These protective groups are strategically employed to allow for controlled and selective reactions during the complex process of peptide chain assembly.
The Fmoc group is renowned for its base-lability, meaning it can be easily removed under mild basic conditions. This characteristic is fundamental to Fmoc-based solid-phase peptide synthesis (SPPS). It permits the sequential addition of amino acids to a growing peptide chain without interfering with the already coupled residues. This orthogonal deprotection strategy is key to building peptides with precise sequences, a requirement for many therapeutic peptides and peptidomimetics.
The tert-butyl group on the serine side chain serves a dual purpose. Firstly, it protects the reactive hydroxyl group from undergoing unwanted side reactions during the coupling steps. Secondly, it enhances the solubility of the amino acid derivative in common organic solvents used in SPPS, facilitating smoother reaction kinetics and improving overall process efficiency. Importantly, the tert-butyl group is typically removed under acidic conditions, often concurrently with the cleavage of the completed peptide from the solid support resin.
The applications of Fmoc-O-tert-butyl-L-serine are extensive and critical in modern scientific research and development. Its primary role is in the synthesis of peptides for various purposes:
1. Peptide-Based Therapeutics: Many novel drugs are peptide-based, targeting diseases ranging from metabolic disorders to cancer. Fmoc-O-tert-butyl-L-serine is a key ingredient in constructing these complex therapeutic peptides, allowing for the introduction of serine residues with protected side chains.
2. Drug Discovery and Development: Researchers utilize this compound to synthesize peptide libraries for high-throughput screening, identifying lead compounds for new drug candidates. The ability to precisely control amino acid incorporation is paramount in this process.
3. Bioconjugation: Fmoc-O-tert-butyl-L-serine can be employed in bioconjugation strategies, where peptides are linked to other molecules, such as antibodies or imaging agents, to create targeted delivery systems or diagnostic tools.
4. Protein Engineering: Modifying proteins to enhance their stability, activity, or specificity often involves peptide synthesis techniques. This serine derivative plays a role in creating engineered proteins with tailored properties.
The purity of Fmoc-O-tert-butyl-L-serine is a critical factor for success in peptide synthesis. Reputable suppliers, such as NINGBO INNO PHARMCHEM CO.,LTD., ensure that their products meet stringent quality standards, often exceeding 98% purity. This high quality guarantees reliable performance, minimizes the risk of producing impure peptides, and saves valuable time and resources.
For scientists and researchers looking to purchase high-quality Fmoc-O-tert-butyl-L-serine, sourcing from a dependable manufacturer in China like NINGBO INNO PHARMCHEM CO.,LTD. offers significant advantages. We are committed to providing the essential tools for scientific advancement, ensuring that your peptide synthesis projects are built on a foundation of exceptional chemical quality. Explore our range of peptide synthesis reagents and experience the difference that quality makes in your research endeavors.
Fmoc-O-tert-butyl-L-serine, also known by its CAS number 71989-33-8, is a derivative of the amino acid L-serine. Its defining features are the Fmoc (9-fluorenylmethyloxycarbonyl) protecting group attached to the alpha-amino group and a tert-butyl ether protecting group on the side chain hydroxyl. These protective groups are strategically employed to allow for controlled and selective reactions during the complex process of peptide chain assembly.
The Fmoc group is renowned for its base-lability, meaning it can be easily removed under mild basic conditions. This characteristic is fundamental to Fmoc-based solid-phase peptide synthesis (SPPS). It permits the sequential addition of amino acids to a growing peptide chain without interfering with the already coupled residues. This orthogonal deprotection strategy is key to building peptides with precise sequences, a requirement for many therapeutic peptides and peptidomimetics.
The tert-butyl group on the serine side chain serves a dual purpose. Firstly, it protects the reactive hydroxyl group from undergoing unwanted side reactions during the coupling steps. Secondly, it enhances the solubility of the amino acid derivative in common organic solvents used in SPPS, facilitating smoother reaction kinetics and improving overall process efficiency. Importantly, the tert-butyl group is typically removed under acidic conditions, often concurrently with the cleavage of the completed peptide from the solid support resin.
The applications of Fmoc-O-tert-butyl-L-serine are extensive and critical in modern scientific research and development. Its primary role is in the synthesis of peptides for various purposes:
1. Peptide-Based Therapeutics: Many novel drugs are peptide-based, targeting diseases ranging from metabolic disorders to cancer. Fmoc-O-tert-butyl-L-serine is a key ingredient in constructing these complex therapeutic peptides, allowing for the introduction of serine residues with protected side chains.
2. Drug Discovery and Development: Researchers utilize this compound to synthesize peptide libraries for high-throughput screening, identifying lead compounds for new drug candidates. The ability to precisely control amino acid incorporation is paramount in this process.
3. Bioconjugation: Fmoc-O-tert-butyl-L-serine can be employed in bioconjugation strategies, where peptides are linked to other molecules, such as antibodies or imaging agents, to create targeted delivery systems or diagnostic tools.
4. Protein Engineering: Modifying proteins to enhance their stability, activity, or specificity often involves peptide synthesis techniques. This serine derivative plays a role in creating engineered proteins with tailored properties.
The purity of Fmoc-O-tert-butyl-L-serine is a critical factor for success in peptide synthesis. Reputable suppliers, such as NINGBO INNO PHARMCHEM CO.,LTD., ensure that their products meet stringent quality standards, often exceeding 98% purity. This high quality guarantees reliable performance, minimizes the risk of producing impure peptides, and saves valuable time and resources.
For scientists and researchers looking to purchase high-quality Fmoc-O-tert-butyl-L-serine, sourcing from a dependable manufacturer in China like NINGBO INNO PHARMCHEM CO.,LTD. offers significant advantages. We are committed to providing the essential tools for scientific advancement, ensuring that your peptide synthesis projects are built on a foundation of exceptional chemical quality. Explore our range of peptide synthesis reagents and experience the difference that quality makes in your research endeavors.
Perspectives & Insights
Agile Reader One
“The ability to precisely control amino acid incorporation is paramount in this process.”
Logic Vision Labs
“Bioconjugation: Fmoc-O-tert-butyl-L-serine can be employed in bioconjugation strategies, where peptides are linked to other molecules, such as antibodies or imaging agents, to create targeted delivery systems or diagnostic tools.”
Molecule Origin 88
“Protein Engineering: Modifying proteins to enhance their stability, activity, or specificity often involves peptide synthesis techniques.”