High-Purity Fmoc-O-tert-butyl-L-serine: Supplier Insights from China
The synthesis of peptides, whether for therapeutic applications, research, or diagnostics, relies heavily on the quality of the constituent building blocks. Among these, Fmoc-O-tert-butyl-L-serine stands out as a frequently used and highly valuable protected amino acid. Understanding the specifications and sourcing reliable supplies are key for researchers. NINGBO INNO PHARMCHEM CO.,LTD., a prominent manufacturer based in China, provides critical insights into why high-purity Fmoc-O-tert-butyl-L-serine is essential and how to procure it effectively.
Fmoc-O-tert-butyl-L-serine, identified by CAS number 71989-33-8, is a derivative of L-serine modified with an N-terminal Fmoc group and a tert-butyl ether protecting group on the serine side chain. These modifications are not arbitrary; they are designed to facilitate complex synthetic procedures, particularly Fmoc-based solid-phase peptide synthesis (SPPS).
Key Specifications for High-Purity Fmoc-O-tert-butyl-L-serine:
When evaluating this reagent, several quality metrics are of utmost importance for researchers aiming for reproducible and successful outcomes:
1. Purity: This is arguably the most critical specification. High-purity Fmoc-O-tert-butyl-L-serine typically exceeds 98% or 99% purity, often determined by High-Performance Liquid Chromatography (HPLC) or chiral HPLC. Impurities can arise from incomplete reactions, side reactions, or degradation, leading to truncated sequences, deleted sequences, or unwanted modifications in the final peptide product. For drug discovery and therapeutic peptide synthesis, exceptionally high purity is non-negotiable.
2. Chiral Purity: As an amino acid derivative, maintaining the correct stereochemistry (L-configuration for L-serine) is vital. Chiral purity, often measured at ≥99%, ensures that the synthesized peptide possesses the intended biological activity. Racemization can occur during synthesis if conditions are not carefully controlled, making chiral purity a key indicator of quality.
3. Appearance: Typically, it is described as a white to off-white powder or crystalline solid. Deviations from this appearance might indicate contamination or degradation.
4. Moisture Content: Low moisture content (often specified as ≤1.0%) is important, as water can interfere with coupling reactions or lead to hydrolysis of protecting groups or the peptide bond itself. NINGBO INNO PHARMCHEM CO.,LTD. ensures that its products are handled and packaged to minimize moisture absorption.
5. Specific Rotation: Optical rotation, such as [α]20/D, serves as another indicator of chiral integrity and purity. A value within the specified range confirms the correct stereochemistry.
Why Source from a Chinese Manufacturer like NINGBO INNO PHARMCHEM CO.,LTD.?
China has become a global hub for chemical manufacturing, offering competitive pricing without compromising quality when sourced from reputable suppliers. NINGBO INNO PHARMCHEM CO.,LTD. leverages its expertise in fine chemical synthesis to provide:
In conclusion, the choice of Fmoc-O-tert-butyl-L-serine and its supplier is a critical decision for any peptide synthesis project. By focusing on high purity, correct chiral integrity, and consistent specifications, researchers can ensure the success of their work. Partnering with established Chinese manufacturers like NINGBO INNO PHARMCHEM CO.,LTD. provides access to these essential reagents, driving innovation in peptide-based research and development.
Fmoc-O-tert-butyl-L-serine, identified by CAS number 71989-33-8, is a derivative of L-serine modified with an N-terminal Fmoc group and a tert-butyl ether protecting group on the serine side chain. These modifications are not arbitrary; they are designed to facilitate complex synthetic procedures, particularly Fmoc-based solid-phase peptide synthesis (SPPS).
Key Specifications for High-Purity Fmoc-O-tert-butyl-L-serine:
When evaluating this reagent, several quality metrics are of utmost importance for researchers aiming for reproducible and successful outcomes:
1. Purity: This is arguably the most critical specification. High-purity Fmoc-O-tert-butyl-L-serine typically exceeds 98% or 99% purity, often determined by High-Performance Liquid Chromatography (HPLC) or chiral HPLC. Impurities can arise from incomplete reactions, side reactions, or degradation, leading to truncated sequences, deleted sequences, or unwanted modifications in the final peptide product. For drug discovery and therapeutic peptide synthesis, exceptionally high purity is non-negotiable.
2. Chiral Purity: As an amino acid derivative, maintaining the correct stereochemistry (L-configuration for L-serine) is vital. Chiral purity, often measured at ≥99%, ensures that the synthesized peptide possesses the intended biological activity. Racemization can occur during synthesis if conditions are not carefully controlled, making chiral purity a key indicator of quality.
3. Appearance: Typically, it is described as a white to off-white powder or crystalline solid. Deviations from this appearance might indicate contamination or degradation.
4. Moisture Content: Low moisture content (often specified as ≤1.0%) is important, as water can interfere with coupling reactions or lead to hydrolysis of protecting groups or the peptide bond itself. NINGBO INNO PHARMCHEM CO.,LTD. ensures that its products are handled and packaged to minimize moisture absorption.
5. Specific Rotation: Optical rotation, such as [α]20/D, serves as another indicator of chiral integrity and purity. A value within the specified range confirms the correct stereochemistry.
Why Source from a Chinese Manufacturer like NINGBO INNO PHARMCHEM CO.,LTD.?
China has become a global hub for chemical manufacturing, offering competitive pricing without compromising quality when sourced from reputable suppliers. NINGBO INNO PHARMCHEM CO.,LTD. leverages its expertise in fine chemical synthesis to provide:
- Cost-Effectiveness: Access to high-quality reagents at competitive prices, allowing research institutions and pharmaceutical companies to optimize their budgets.
- Scalability: The capability to supply materials from grams to kilograms, supporting projects from early research through to pilot-scale production.
- Reliability: A commitment to stringent quality control processes, ensuring batch-to-batch consistency and reliable supply chains. If you need to buy Fmoc-O-tert-butyl-L-serine, our consistent quality is a primary benefit.
- Technical Support: Access to expertise regarding product specifications, handling, and application, aiding researchers in optimizing their synthetic strategies.
In conclusion, the choice of Fmoc-O-tert-butyl-L-serine and its supplier is a critical decision for any peptide synthesis project. By focusing on high purity, correct chiral integrity, and consistent specifications, researchers can ensure the success of their work. Partnering with established Chinese manufacturers like NINGBO INNO PHARMCHEM CO.,LTD. provides access to these essential reagents, driving innovation in peptide-based research and development.
Perspectives & Insights
Future Origin 2025
“High-purity Fmoc-O-tert-butyl-L-serine typically exceeds 98% or 99% purity, often determined by High-Performance Liquid Chromatography (HPLC) or chiral HPLC.”
Core Analyst 01
“Impurities can arise from incomplete reactions, side reactions, or degradation, leading to truncated sequences, deleted sequences, or unwanted modifications in the final peptide product.”
Silicon Seeker One
“For drug discovery and therapeutic peptide synthesis, exceptionally high purity is non-negotiable.”