Precise protein modification is a cornerstone of modern molecular biology and biochemical research. N-Succinimidyl Bromoacetate (CAS 42014-51-7) emerges as a highly effective reagent for achieving specific protein labeling, enabling scientists to study protein functions, interactions, and localization with greater accuracy. This heterobifunctional crosslinker provides a versatile platform for attaching various molecular tags, such as fluorescent dyes, biotin, or affinity ligands, to target proteins.

The mechanism behind N-Succinimidyl Bromoacetate's utility in protein labeling lies in its dual reactive ends. The succinimidyl ester moiety is designed to react with the epsilon-amino groups of lysine residues or the N-terminal alpha-amino group of a protein. This reaction, typically performed at a slightly alkaline pH (6.5-8.5), results in the formation of a stable amide bond, effectively labeling the protein with the bromoacetyl group. Subsequently, the bromoacetyl group can be employed to react with sulfhydryl groups, often present in cysteine residues, creating a thioether linkage. This step-wise approach allows for controlled and specific labeling, minimizing unwanted side reactions.

For researchers and procurement managers looking to buy N-Succinimidyl Bromoacetate, understanding its applications in protein labeling is key. It is widely used for attaching fluorescent probes for microscopy and flow cytometry, for conjugating proteins to surfaces in ELISA assays or protein arrays, and for labeling proteins with biotin for subsequent detection or purification via streptavidin-based systems. The consistent purity of the reagent, usually a white powder with high NMR purity, is critical to ensure the specificity and efficiency of the labeling process.

When sourcing this important chemical, especially from manufacturers in China, it is vital to partner with reliable suppliers who guarantee quality and timely delivery. Price considerations should be balanced with the assurance of high purity and proper handling instructions, such as storage at freezer temperatures. A trusted manufacturer will provide detailed product specifications, including the CAS number 42014-51-7, and offer support for custom packaging or bulk orders.

In essence, N-Succinimidyl Bromoacetate offers a robust solution for researchers needing to precisely label proteins. By selecting a reputable supplier that emphasizes product quality and technical support, scientists can confidently integrate this reagent into their experimental workflows, advancing their understanding of complex biological systems and developing novel biotechnological applications.