In the realm of analytical chemistry, precision and sensitivity are key. For researchers and laboratory technicians focused on amino acid analysis, particularly using High-Performance Liquid Chromatography (HPLC), the choice of derivatization reagent significantly impacts the quality of results. 9-Oxo-10(9H)-Acridineacetic Acid (CAS 38609-97-1) emerges as a highly effective reagent for pre-column derivatization, transforming non-fluorescent amino acids into fluorescent derivatives that are ideal for sensitive detection. This article explores its utility and guides on how to source this vital analytical tool.

The Advantage of Fluorescent Derivatization

Amino acids, the fundamental building blocks of proteins, often lack inherent fluorescence, making their direct detection by fluorescence detectors in HPLC challenging. Pre-column derivatization involves reacting the amino acids with a fluorescent labeling agent before injection into the HPLC system. This process converts them into highly fluorescent compounds, dramatically increasing sensitivity and enabling accurate quantification even at very low concentrations.

9-Oxo-10(9H)-Acridineacetic Acid excels in this role. Its reaction with amino acids yields stable fluorescent derivatives. This makes it an invaluable tool for various applications, including nutritional analysis, clinical diagnostics, quality control in the food and pharmaceutical industries, and fundamental biochemical research. When you buy 9-Oxo-10(9H)-Acridineacetic Acid, you are investing in improved analytical performance.

Key Benefits for HPLC Users

  • Enhanced Sensitivity: Significantly boosts the detection limits for amino acids, allowing for the analysis of trace amounts.
  • Improved Specificity: The fluorescent tags can offer better selectivity, reducing interference from other sample components.
  • Reliable Reaction: Forms stable derivatives, ensuring reproducibility in analytical workflows.
  • Broad Applicability: Works effectively with a wide range of common amino acids.

Where to Source High-Quality Reagents

To ensure optimal performance in your HPLC analyses, it is crucial to obtain 9-Oxo-10(9H)-Acridineacetic Acid from a reputable supplier. Look for manufacturers who specialize in analytical reagents and fine chemicals. Key factors to consider when making a purchase decision include:

  • Purity: Typically, a purity of ≥99.0% (HPLC grade) is recommended for derivatization reagents to avoid introducing impurities that could affect chromatograms.
  • Supplier Reputation: Partner with companies known for consistent quality and reliable supply chains. A good manufacturer will provide comprehensive product documentation, including COAs and SDS.
  • Availability and Pricing: Compare offers from different suppliers to find competitive price points, especially for bulk purchases. Many suppliers offer various pack sizes to suit different laboratory needs.

When seeking to purchase this reagent, consider approaching dedicated chemical suppliers who understand the stringent requirements of analytical laboratories. They can often provide detailed technical support and ensure the product meets the necessary specifications for your HPLC setup.

Conclusion

For laboratories aiming to achieve superior amino acid analysis via HPLC, 9-Oxo-10(9H)-Acridineacetic Acid is an excellent choice for fluorescent derivatization. Its ability to enhance sensitivity and reliability makes it a critical component in many analytical workflows. By sourcing this reagent from trusted manufacturers and suppliers, you can ensure the accuracy and efficiency of your research and quality control processes.