The field of nucleic acid chemistry has witnessed transformative advancements, largely driven by the ability to synthesize modified oligonucleotides with tailored properties. These modifications are crucial for overcoming the inherent limitations of natural DNA and RNA, paving the way for innovative applications in medicine and biotechnology. Among the most impactful modifications is the 2'-O-methylation of ribonucleosides, and the phosphoramidites that enable this modification are of paramount importance.

This article focuses on the benefits derived from using 2'-O-methyl cytidine phosphoramidites, specifically exemplified by N-Acetyl-5'-O-(4,4-dimethoxytrityl)-2'-O-methylcytidine-3'-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite (CAS 199593-09-4).

Understanding the 2'-O-Methyl Modification:

In RNA, the presence of a hydroxyl group at the 2' position of the ribose sugar makes it susceptible to hydrolysis and enzymatic cleavage. The 2'-O-methyl modification replaces this reactive hydroxyl group with a stable methyl group. This alteration yields several significant advantages:

  • Enhanced Stability Against Nucleases: The steric bulk introduced by the methyl group effectively shields the phosphodiester backbone from enzymatic attack by RNases and DNases. This translates to an extended in vivo half-life for therapeutic oligonucleotides and improved reliability for diagnostic probes.
  • Increased Duplex Melting Temperature (Tm): Oligonucleotides containing 2'-O-methyl modifications exhibit higher binding affinity to their complementary target sequences. This increase in Tm means that duplexes formed are more stable, leading to enhanced specificity and sensitivity in assays and therapeutic interventions.
  • Improved Chemical Stability: Beyond enzymatic resistance, 2'-O-methylated RNA also shows improved chemical stability compared to unmodified RNA, further contributing to its robustness in various applications.
  • Compatibility with Standard Synthesis: Crucially, the 2'-O-methyl group is stable under standard oligonucleotide synthesis and deprotection conditions. This means that these modified nucleotides can be seamlessly incorporated into the automated synthesis workflow using standard phosphoramidite chemistry, making them readily accessible for researchers and manufacturers.

Applications and Procurement:

The benefits of 2'-O-methyl cytidine phosphoramidites are widely leveraged in the development of:

  • Antisense Oligonucleotides (ASOs) and siRNAs: To achieve sustained gene silencing and therapeutic effects.
  • Aptamers: For their use as molecular recognition agents in diagnostics and therapeutics.
  • Diagnostic Probes: For highly sensitive and specific detection in PCR, qPCR, and FISH assays.

As a dedicated manufacturer and supplier, we offer high-purity N-Acetyl-5'-O-(4,4-dimethoxytrityl)-2'-O-methylcytidine-3'-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite. We understand the importance of reliable sourcing for these advanced materials. If you are looking to buy this essential building block to enhance your oligonucleotide designs, contact us for competitive pricing and dependable supply from China. Our commitment to quality ensures you receive the consistent performance required for cutting-edge research and development.