The synthesis of peptides, vital molecules for biological functions and therapeutic applications, demands precision at every step. Central to this process is the strategic use of protected amino acids, ensuring that each building block is correctly incorporated into the growing peptide chain. Among these, Fmoc-Ser(tBu)-OH emerges as a critical reagent, particularly for introducing serine residues within the framework of Fmoc solid-phase peptide synthesis (SPPS).

Fmoc-Ser(tBu)-OH, identified by its CAS Number 71989-33-8, is a derivative of L-serine where both the alpha-amino group and the side-chain hydroxyl group are protected. The N-alpha-amino group is protected by the fluorenylmethoxycarbonyl (Fmoc) group. This protecting group is labile to mild bases, allowing for its selective removal by reagents such as piperidine, which is a standard procedure in Fmoc SPPS. This deprotection step exposes the free amino group of the terminal amino acid, making it ready for the next coupling reaction.

The side chain of serine possesses a hydroxyl (-OH) group, which is nucleophilic and can interfere with peptide bond formation or undergo undesired modifications during synthesis. To circumvent this, Fmoc-Ser(tBu)-OH utilizes a tert-butyl (tBu) ether protection for the hydroxyl group. The tert-butyl group is bulky and electron-donating, providing excellent protection during the repetitive cycles of Fmoc deprotection and amino acid coupling. Critically, the tBu ether linkage is stable under the basic conditions used for Fmoc removal. It is, however, readily cleaved by strong acids, typically in a cocktail containing scavengers, during the final step of peptide cleavage from the resin and concomitant deprotection of other acid-labile side-chain protecting groups.

This dual protection strategy makes Fmoc-Ser(tBu)-OH an indispensable tool for peptide chemists. Its use ensures that serine residues are incorporated cleanly, and the side-chain hydroxyl group remains untouched until the final cleavage step. This leads to higher fidelity in peptide sequences and simplifies downstream purification. As a premier Fmoc-amino acid supplier, we are committed to providing Fmoc-Ser(tBu)-OH with exceptional purity, ensuring that our clients, whether they are researchers or pharmaceutical manufacturers, can achieve optimal results in their peptide synthesis projects.

When sourcing this essential reagent, understanding its specifications is key. Fmoc-Ser(tBu)-OH is typically supplied as a white to off-white powder, with a molecular weight of 383.44 g/mol. The quality and consistency of the material are crucial for reproducible peptide synthesis. For those looking to buy Fmoc-Ser(tBu)-OH, especially in larger quantities for industrial applications, engaging with a reliable Fmoc-Ser(tBu)-OH manufacturer is advisable. Exploring Fmoc-Ser(tBu)-OH price points from reputable vendors will help in budgeting for peptide synthesis projects.

In conclusion, Fmoc-Ser(tBu)-OH is not just another protected amino acid; it is a carefully engineered building block that underpins the successful incorporation of serine in complex peptides synthesized via Fmoc SPPS. Its reliable performance makes it a go-to reagent for peptide synthesis. We stand ready as your trusted supplier to provide high-quality Fmoc-Ser(tBu)-OH to meet all your peptide synthesis needs.