Solid-phase peptide synthesis (SPPS) remains a cornerstone of modern peptide chemistry, enabling the creation of complex and biologically active peptides. At the heart of a successful SPPS campaign lies the quality of the resins and building blocks used. Among these, Fmoc-Asn(Trt)-Wang Resin stands out as a critical component for incorporating the asparagine residue, a common amino acid with a potentially problematic side chain that requires careful protection.

Fmoc-Asn(Trt)-Wang Resin utilizes the Nα-Fmoc (9-fluorenylmethyloxycarbonyl) protection strategy, which is base-labile and widely adopted for its orthogonality. The asparagine side chain is protected with a trityl (Trt) group. This protection is crucial because the amide in the asparagine side chain can be prone to side reactions, such as dehydration to a nitrile or aspartimide formation, under certain coupling or deprotection conditions. The bulky trityl group effectively shields the amide, preventing these undesirable reactions and ensuring the integrity of the growing peptide chain.

The Wang resin itself is a polystyrene resin substituted with a 4-hydroxymethylphenyl linker. This linker is stable under Fmoc deprotection conditions but is readily cleaved by strong acids, typically trifluoroacetic acid (TFA), releasing the C-terminal peptide. The choice of resin support and linker is vital for efficient synthesis and clean cleavage. When looking to buy Fmoc-Asn(Trt)-Wang resin, it is essential to consider specifications such as loading capacity and particle size. Common loading capacities range from 0.3 to 0.8 mmol/g, which dictates how much peptide can be synthesized from a given amount of resin. Particle size, often specified in mesh (e.g., 100-200 mesh or 200-400 mesh), influences swelling and diffusion rates during synthesis.

For researchers and procurement managers seeking a reliable source, identifying a reputable Fmoc-Asn(Trt)-Wang resin manufacturer in China can offer significant advantages in terms of cost-effectiveness and supply chain stability. Companies specializing in peptide synthesis reagents often provide detailed technical specifications, batch-specific certificates of analysis, and robust quality control. This ensures that the resin meets the stringent demands of pharmaceutical intermediate production and advanced research projects. When comparing prices, it's important to factor in purity, consistency, and supplier support. A clear Fmoc-Asn(Trt)-Wang resin price quote should reflect these quality parameters.

Successful implementation of Fmoc-Asn(Trt)-Wang Resin involves careful consideration of coupling reagents, solvents, and deprotection steps. For instance, highly efficient coupling reagents like HATU or HBTU are often employed. Deprotection of the Fmoc group is typically achieved using a solution of piperidine in DMF. Post-synthesis, the peptide is cleaved from the resin using a cocktail of TFA and scavengers to remove the side-chain protecting groups and prevent side reactions with reactive intermediates. Understanding these parameters is key to achieving high yields and purity. If you are looking to purchase Fmoc-Asn(Trt)-Wang resin for your next peptide synthesis project, partnering with a trusted Fmoc-Asn(Trt)-Wang resin supplier will ensure the quality and success of your work.