Understanding BCIP p-Toluidine Salt: Properties and Applications in Detection
BCIP p-toluidine salt, also known as 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt (CAS 6578-06-9), is a cornerstone reagent in numerous biochemical and molecular biology applications. Its primary function is to act as a chromogenic substrate for the enzyme alkaline phosphatase (AP). This substrate is instrumental in visually detecting the presence and activity of AP-conjugated antibodies or nucleic acid probes.
The chemical mechanism involves the enzymatic dephosphorylation of BCIP p-toluidine salt by alkaline phosphatase. This reaction liberates an indoxyl derivative which, upon subsequent oxidation (often facilitated by agents like Nitro Blue Tetrazolium chloride - NBT), forms a distinct, insoluble blue precipitate. This colorimetric detection method is highly valued for its sensitivity and straightforward visualization, making it a preferred choice in techniques such as Western blotting, immunohistochemistry (IHC), and Southern/Northern blotting.
Beyond its role in detection assays, BCIP p-toluidine salt is also recognized for its significance as a chemical intermediate. In the pharmaceutical industry, it serves as a crucial building block in the synthesis of complex molecules. Similarly, its utility extends to the agrochemical sector, where it can be employed as an intermediate in the production of specialized compounds. The consistent availability of high-purity BCIP p-toluidine salt (often specified at 99% minimum assay) from reliable Chinese suppliers is critical for ensuring the quality and reproducibility of these processes.
For laboratories and research institutions, understanding the handling and application of BCIP p-toluidine salt is key to maximizing its potential. Its solubility in solvents like DMF and its typical presentation as a white to off-white powder are important physical properties to consider. When sourcing this reagent, partnering with a reputable supplier in China offers the dual benefit of competitive pricing and assurance of quality. Whether you are performing intricate molecular detection or synthesizing vital intermediates, the reliability of your chemical supply chain directly impacts your success.
The widespread adoption of BCIP p-toluidine salt in scientific research highlights its enduring importance. As a vital chromogenic phosphatase substrate and a valuable chemical intermediate, its role continues to expand across various scientific disciplines.
The chemical mechanism involves the enzymatic dephosphorylation of BCIP p-toluidine salt by alkaline phosphatase. This reaction liberates an indoxyl derivative which, upon subsequent oxidation (often facilitated by agents like Nitro Blue Tetrazolium chloride - NBT), forms a distinct, insoluble blue precipitate. This colorimetric detection method is highly valued for its sensitivity and straightforward visualization, making it a preferred choice in techniques such as Western blotting, immunohistochemistry (IHC), and Southern/Northern blotting.
Beyond its role in detection assays, BCIP p-toluidine salt is also recognized for its significance as a chemical intermediate. In the pharmaceutical industry, it serves as a crucial building block in the synthesis of complex molecules. Similarly, its utility extends to the agrochemical sector, where it can be employed as an intermediate in the production of specialized compounds. The consistent availability of high-purity BCIP p-toluidine salt (often specified at 99% minimum assay) from reliable Chinese suppliers is critical for ensuring the quality and reproducibility of these processes.
For laboratories and research institutions, understanding the handling and application of BCIP p-toluidine salt is key to maximizing its potential. Its solubility in solvents like DMF and its typical presentation as a white to off-white powder are important physical properties to consider. When sourcing this reagent, partnering with a reputable supplier in China offers the dual benefit of competitive pricing and assurance of quality. Whether you are performing intricate molecular detection or synthesizing vital intermediates, the reliability of your chemical supply chain directly impacts your success.
The widespread adoption of BCIP p-toluidine salt in scientific research highlights its enduring importance. As a vital chromogenic phosphatase substrate and a valuable chemical intermediate, its role continues to expand across various scientific disciplines.
Perspectives & Insights
Agile Reader One
“Whether you are performing intricate molecular detection or synthesizing vital intermediates, the reliability of your chemical supply chain directly impacts your success.”
Logic Vision Labs
“The widespread adoption of BCIP p-toluidine salt in scientific research highlights its enduring importance.”
Molecule Origin 88
“As a vital chromogenic phosphatase substrate and a valuable chemical intermediate, its role continues to expand across various scientific disciplines.”