In the realm of biological research, accurately assessing cell health and viability is paramount. Among the array of tools available, Neutral Red (CAS 553-24-2) stands out as a remarkably effective and widely utilized dye for cellular assays. As a leading chemical supplier, we understand the critical need for high-quality reagents, and Neutral Red fits this description perfectly. Its unique properties make it an indispensable component in numerous research protocols, particularly in the fields of toxicology and drug discovery.

Neutral Red, also known as Basic Red 5, is a cationic dye belonging to the oxazine class. It is characterized by its distinctive color and its ability to permeate the plasma membrane of living cells through passive diffusion. Once inside the cell, it is actively sequestered and accumulated within the lysosomes, which are organelles containing hydrolytic enzymes and maintaining an acidic internal pH. This specific accumulation mechanism is what makes Neutral Red an excellent vital stain for determining cell viability. Researchers frequently buy Neutral Red for its reliability in quantifying cellular uptake and retention, which directly correlates with cell membrane integrity and lysosomal function.

The most common application where researchers purchase Neutral Red is the Neutral Red Uptake (NRU) assay. In this assay, cells are incubated with a specific concentration of Neutral Red solution. Viable cells with intact lysosomes efficiently take up and retain the dye. Non-viable or damaged cells, on the other hand, exhibit reduced or no uptake, or they may leak the dye out. After a suitable incubation period, the dye is extracted from the cells using a solvent (like ethanol or DMSO), and the absorbance is measured spectrophotometrically. A higher absorbance value indicates more dye uptake, signifying greater cell viability. This makes Neutral Red a quantitative measure of a cell's metabolic activity and its capacity to maintain a functional lysosomal system. When you need to buy this assay component, sourcing from a reputable chemical manufacturer ensures the purity and consistency required for reproducible results.

Beyond cell viability, Neutral Red also serves as a pH indicator. Its color changes from red in neutral to alkaline conditions (pH 6.8-8.0) to yellow in acidic conditions. This property can be leveraged in specific experimental setups, although its primary utility remains in cellular staining and viability assessments. For researchers looking to procure this versatile chemical, understanding its applications and sourcing from a trusted manufacturer is key. We are dedicated to supplying high-purity Neutral Red to research institutions and laboratories worldwide, ensuring that you can confidently purchase the materials needed for your groundbreaking studies. Consider us your primary supplier for all your laboratory chemical needs, including essential stains like Neutral Red.