In the realm of scientific research, maintaining precise pH levels is often paramount to the success of experiments. Sodium Citrate Dihydrate (CAS 68-04-2) stands out as a highly effective buffering agent, offering stability across a range of pH values crucial for biological and chemical assays. Understanding its role and sourcing it from reputable suppliers is key for any research laboratory.

At its core, Sodium Citrate is a salt derived from citric acid. In aqueous solutions, it dissociates into sodium ions and citrate ions. The citrate ion is a weak base that can accept protons (H+) or donate protons, thereby resisting significant changes in pH when acids or bases are introduced. This characteristic makes it an indispensable tool in biochemical assays, enzyme studies, and molecular biology applications where specific pH environments are critical for optimal reaction rates and protein stability.

For instance, in applications like immunohistochemistry, citrate buffers are frequently used for antigen retrieval. This process involves heating tissue samples in a citrate buffer solution to break the cross-links formed during fixation, thereby unmasking the target antigens for antibody binding. The reliability of the buffer's pH is essential to ensure consistent staining intensity and avoid damage to the tissue morphology.

Researchers frequently utilize Sodium Citrate solutions for various purposes, including electrophoresis, cell culture media preparation, and DNA/RNA handling. The ability to purchase Sodium Citrate in bulk from a trusted manufacturer ensures that laboratories can maintain a consistent supply of high-quality buffering agents without compromising experimental integrity. When searching for a supplier, consider their commitment to quality control and the availability of detailed technical specifications.

The choice of buffer concentration and pH is dictated by the specific experimental requirements. Whether you need a solution for a delicate enzyme assay or a robust protocol for tissue processing, reliable Sodium Citrate is fundamental. For laboratories in China and worldwide, partnering with experienced chemical manufacturers provides access to consistent product quality and competitive pricing, ensuring that research objectives can be met efficiently and accurately.