In the expansive realm of organic chemistry and biochemistry, certain compounds stand out for their fundamental significance in unraveling complex biological processes. Among these is (S)-2-((S)-2-Amino-4-methylpentanamido)-4-methylpentanoic Acid, widely known by its more concise name, H-Leu-Leu-OH, and identified by CAS Number 3303-31-9. This remarkable compound is a dipeptide, specifically formed from two L-leucine residues, making it an invaluable tool for a wide array of scientific investigations.

H-Leu-Leu-OH possesses a molecular formula of C12H24N2O3 and a molecular weight of 244.33 g/mol. Typically presenting as a white solid or powder, its physical characteristics, including a melting point around 270-272°C (with decomposition), are well-defined. While it exhibits slight solubility in water and methanol when heated or sonicated, its hydrophobic nature due to the leucine side chains influences its overall solubility profile. These properties are critical for researchers when designing experiments and formulating solutions in their work.

The applications of H-Leu-Leu-OH are diverse and impactful, particularly in the fields of biochemistry and molecular biology. As a neutral dipeptide derivative, it is extensively utilized to delve into the functionality of dileucine motifs. These motifs are vital in various cellular mechanisms, including the internalization and precise targeting of proteins, such as the vesicular acetylcholine transporter, and their interactions with clathrin adaptors like AP-1 and AP-2. Furthermore, H-Leu-Leu-OH plays a significant role in studying protein synthesis and cellular signaling pathways. Its utility extends to acting as a substrate for dipeptidase activity measurement in various biological samples, including serum, tissues, and urine, providing insights into metabolic processes. Researchers also leverage this compound as an experimental model for biological studies, including investigations into cell lysis and apoptosis, underscoring its versatility as a research chemical.

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