4,5-Dicyanoimidazole (DCI): A Superior Activator for Enhanced Oligonucleotide Synthesis
Unlock higher yields and efficiency in your DNA and RNA synthesis with this advanced chemical activator.
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4,5-Dicyanoimidazole
4,5-Dicyanoimidazole (DCI) stands out as a highly effective activator for oligonucleotide synthesis. It significantly enhances the coupling efficiency between phosphoramidites and the growing oligonucleotide chain, a critical step for successful synthesis. Unlike traditional activators such as tetrazole, DCI exhibits superior nucleophilicity and reduced acidity, addressing key limitations in current methodologies. This makes it an ideal choice for researchers seeking to improve yields and achieve greater success in complex synthesis projects, including challenging RNA sequences where other activators may falter.
- Discover the advantages of using DCI for oligonucleotide synthesis, a key to improving coupling efficiency in DNA synthesis.
- Explore 4,5-dicyanoimidazole as a superior alternative to tetrazole-based activators for enhanced oligonucleotide synthesis outcomes.
- Learn how DCI can facilitate challenging RNA synthesis processes, leading to better yields and product purity.
- Optimize your chemical synthesis workflows with DCI, ensuring more reliable and efficient production of oligonucleotide chains.
Key Product Advantages
Superior Coupling Efficiency
DCI demonstrably doubles the coupling rate compared to tetrazole, directly impacting the overall yield of your oligonucleotide synthesis. This boost in efficiency is crucial for obtaining high-quality, full-length products, particularly when aiming for enhanced oligonucleotide synthesis.
Reduced Acidity and Side Reactions
With a pKa of 5.2, DCI is less acidic than tetrazole (pKa 4.8). This lower acidity minimizes unwanted side reactions, such as trityl group deprotection, which can lead to dimer formation and lower yields in your DNA synthesis experiments.
Enhanced Solubility and Workability
DCI boasts better solubility in acetonitrile, allowing for higher concentration solutions. This improved solubility, coupled with its potent activity, makes it a more versatile and manageable reagent for various synthesis scales, supporting your efforts in chemical synthesis.
Key Applications
DNA Synthesis
Leverage DCI to achieve significantly improved coupling efficiencies in the synthesis of DNA strands, ensuring higher purity and yield for your research projects. This is vital for DNA synthesis advancements.
RNA Synthesis
DCI is particularly effective in RNA synthesis, overcoming the challenges posed by bulky protecting groups and contributing to the successful production of complex RNA molecules through RNA synthesis optimization.
Oligonucleotide Synthesis
As a premier activator, DCI enhances the overall performance of solid-phase oligonucleotide synthesis, providing a reliable solution for producing both standard and modified oligonucleotides by utilizing this DCI oligonucleotide synthesis reagent.
Custom Synthesis Projects
For specialized chemical synthesis needs, DCI offers a robust and efficient activation pathway, supporting the creation of novel nucleic acid structures and derivatives through advanced chemical synthesis methodologies.