Bicine: A Versatile Biological Buffer for Biochemical Research and Applications
Explore the essential role of Bicine in maintaining stable pH for critical biochemical processes.
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Bicine
Bicine (CAS No: 150-25-4) is a highly effective zwitterionic organic compound that serves as a crucial biological buffer. Its primary function is to maintain stable pH levels, which is paramount for the accurate execution of various biochemical reactions and research protocols. It is particularly noted for its utility in biochemical research conducted at low temperatures, and for its role in preparing stable substrate solutions essential for specific analytical determinations. The compound is also recognized for enhancing protein stability and improving chromatography yields, making it invaluable in molecular biology applications.
- Explore the benefits of using Bicine for biochemical research conducted at low temperatures. This specific application highlights its suitability for sensitive experiments requiring precise environmental control.
- Learn how to prepare a stable substrate solution using Bicine, a key process for many biochemical assays and analytical procedures.
- Discover the advantages of Bicine in protein crystallization and its contribution to increased ion exchange chromatography yields.
- Understand Bicine's purity specifications, with assays typically registering ≥99.0%, ensuring reliability in demanding scientific applications.
Key Advantages
Stable pH Control
Bicine provides reliable pH maintenance within its working range, critical for enzyme function and reaction kinetics in biochemical research.
Low-Temperature Efficacy
Its utility in low-temperature biochemical research underscores its robustness and effectiveness across a broader spectrum of experimental conditions.
Enhanced Protein Stability
By using Bicine, researchers can significantly enhance protein stability, which is vital for obtaining accurate results in protein crystallization and other related studies.
Key Applications
Biochemical Research
Bicine is widely used as a biological buffer in various biochemical research settings, ensuring consistent pH for sensitive experiments.
Protein Stabilization
Researchers utilize Bicine to improve protein stability during purification and storage, crucial for its protein crystallization applications.
Chromatography
The compound aids in ion exchange chromatography, contributing to higher yields and purity of separated proteins.
Assay Development
Preparing stable substrate solutions with Bicine is a common practice in assay development, ensuring reproducibility of results.