High-Purity 5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal) for Biological Assays and Gene Expression Studies
Discover the power of X-Gal, the gold standard chromogenic substrate for detecting beta-galactosidase activity, essential for precise gene expression analysis and robust molecular cloning.
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5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside
As a leading supplier in China, we provide high-purity 5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal), a critical chromogenic substrate for beta-galactosidase detection. This compound is indispensable for various biotechnological applications, including precise blue-white screening in molecular cloning and the sensitive detection of gene expression. Our commitment to quality ensures that researchers worldwide receive a reliable product for their demanding experiments.
- Leverage our high-purity X-Gal for accurate blue-white screening in molecular cloning experiments, a key aspect of successful gene reporter assays.
- Utilize this essential chromogenic substrate to visualize and quantify beta-galactosidase activity, aiding in the understanding of gene expression patterns.
- Incorporate X-Gal into your molecular biology workflows for reliable identification of recombinant plasmids, ensuring the integrity of your genetic constructs.
- Benefit from a trusted manufacturer in China, providing consistent quality for critical enzyme assays and other biochemical research applications.
Advantages of Using X-Gal
Precise Detection of Beta-galactosidase
Our high-purity X-Gal guarantees reliable and sensitive detection of beta-galactosidase activity, crucial for accurate gene expression studies.
Facilitates Blue-White Screening
It's a fundamental tool for molecular cloning, enabling clear visual identification of recombinant colonies through its characteristic blue color formation.
Versatile Research Applications
Beyond cloning, X-Gal is vital for various enzyme assays, microbiology, and diagnostics, supporting a broad range of scientific inquiries.
Key Applications
Molecular Cloning
Essential for blue-white screening to identify successful gene insertions and recombinant plasmids in cloning experiments.
Gene Expression Analysis
Used as a reporter gene assay to monitor and quantify the activity of the lacZ gene or other beta-galactosidase fusion proteins.
Microbiology
Aids in the detection and differentiation of microorganisms expressing beta-galactosidase, such as in E. coli identification.
Biochemical Assays
Serves as a chromogenic substrate in various enzymatic assays where beta-galactosidase activity needs to be measured.