Y-27632 Dihydrochloride: A Potent ROCK Inhibitor for Cell Culture and Biomedical Research
Unlock groundbreaking research with a highly selective ROCK inhibitor, enhancing stem cell survival and reprogramming.
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ROCK Inhibitor Y-27632 Dihydrochloride
Y-27632 dihydrochloride is a cell-permeable, highly potent, and selective inhibitor of Rho-associated protein kinases (ROCK1 and ROCK2). It plays a crucial role in enhancing stem cell survival and cloning efficiency, particularly during dissociation processes, and is vital for improving embryoid body formation and cryopreservation success.
- Enhance stem cell survival: Utilize Y-27632 dihydrochloride to significantly improve the viability of human embryonic stem cells when dissociated into single cells, preventing anoikis and boosting cloning efficiency. This is a key application for stem cell enhancement technologies.
- Facilitate reprogramming and differentiation: This compound is instrumental in various cell reprogramming and differentiation protocols, including the direct conversion of fibroblasts into mature neurons, showcasing its utility in cell reprogramming agent applications.
- Improve cryopreservation: Incorporate Y-27632 dihydrochloride as a cryopreservation media component to increase the survival rate of human embryonic stem (hES) cells and induced pluripotent stem cells (iPSCs) after thawing.
- Advance biomedical research: As a critical biomedical research chemical, Y-27632 dihydrochloride supports a wide range of studies in cell biology, developmental biology, and drug discovery, offering a reliable Rho-associated kinase inhibitor for experimental use.
Key Advantages
Unparalleled Selectivity
Achieve precise experimental outcomes with a highly selective inhibitor of ROCK1 and ROCK2, minimizing off-target effects in your research, crucial for targeted kinase inhibition in research.
Enhanced Cell Viability
Improve the success rate of your cell culture experiments by ensuring superior cell survival and adherence, making it an indispensable tool in advanced cell culture reagents.
Versatile Application Spectrum
From reprogramming to differentiation and cryopreservation, Y-27632 dihydrochloride offers a broad range of applications, supporting diverse research needs in biomedical applications of small molecules.
Key Applications
Stem Cell Maintenance and Expansion
Crucial for maintaining the pluripotency and enhancing the survival of human embryonic stem cells and iPSCs during dissociation and passaging, a cornerstone of stem cell enhancement technologies.
Reprogramming and Differentiation Studies
A key component in protocols for direct lineage reprogramming and directed differentiation, enabling the generation of specific cell types for research, as seen in cell reprogramming agent applications.
Cryopreservation of Stem Cells
Significantly improves the post-thaw recovery and viability of stem cells, making it an essential cryopreservation media component for preserving valuable cell lines.
Drug Discovery and Screening
Utilized in various drug discovery workflows to investigate cellular pathways regulated by ROCK, contributing to the development of novel therapeutics and supporting biomedical applications of small molecules.