H-D-Ala-pNA HCl (CAS: 201731-77-3), or D-Alanine 4-nitroanilide hydrochloride, is a precisely engineered molecule with specific chemical properties that make it invaluable in scientific research, particularly in enzymology and microbiology. Understanding its structure and synthesis is key to appreciating its function.

Chemically, H-D-Ala-pNA HCl is an amino acid derivative where D-alanine is linked via an amide bond to 4-nitroaniline. The hydrochloride salt form ensures greater solubility and stability. Its molecular formula is C9H11N3O3·HCl, with a molecular weight of approximately 245.66 g/mol. The presence of the nitro group on the phenyl ring is critical for its chromogenic properties; it's the electronic configuration of this nitro group that allows the liberated 4-nitroaniline to absorb light in the visible spectrum, resulting in a yellow color.

The synthesis of H-D-Ala-pNA HCl typically involves coupling D-alanine with 4-nitroaniline. This amide bond formation is often facilitated by coupling agents such as carbodiimides (e.g., DCC or EDC) in an appropriate organic solvent, like dimethylformamide (DMF) or dichloromethane (DCM). Following the coupling reaction, purification steps, which may include recrystallization or chromatography, are employed to isolate the pure product. The final product is then typically converted to its hydrochloride salt.

The key properties that make H-D-Ala-pNA HCl a research essential include:

  • Chromogenic nature: Its ability to yield a colored product upon enzymatic hydrolysis is fundamental to its use in assays.
  • Specificity: It is designed to be a substrate for D-aminopeptidases, offering specificity in enzymatic studies.
  • Solubility and Stability: As a hydrochloride salt, it generally exhibits good solubility in aqueous buffers and sufficient stability for laboratory use.
  • Molecular Weight: Its defined molecular weight aids in stoichiometric calculations for assay development.

NINGBO INNO PHARMCHEM CO.,LTD. is committed to providing researchers with compounds of the highest purity and precise specifications. Our H-D-Ala-pNA HCl is synthesized and purified rigorously to meet the demanding requirements of scientific research. We understand that the quality of reagents directly impacts the validity of experimental results.

By focusing on the chemical integrity and reliable synthesis of compounds like H-D-Ala-pNA HCl, NINGBO INNO PHARMCHEM CO.,LTD. supports the advancement of biochemical research and diagnostics. When you choose NINGBO INNO PHARMCHEM CO.,LTD., you are choosing a partner dedicated to quality and scientific progress.