Biotin-NHS Ester is a versatile chemical compound that has become indispensable in various life science research disciplines, primarily for its role in labeling biomolecules. As a leading supplier, we provide researchers with high-purity Biotin-NHS Ester, enabling a wide array of applications that drive scientific advancement.

The fundamental utility of Biotin-NHS Ester lies in its reactivity with primary amine groups found in biomolecules. This amine-reactive functional group allows for the efficient covalent attachment of biotin to proteins, peptides, antibodies, and other amine-containing molecules. Once biotinylated, these molecules can be readily detected or purified using the strong and specific interaction between biotin and streptavidin or avidin. This forms the basis for many analytical techniques used in molecular biology and biochemistry.

One of the most common applications is protein labeling. Researchers utilize Biotin-NHS Ester to label antibodies, enzymes, and other proteins. This biotinylated protein can then be used in conjunction with labeled avidin or streptavidin conjugates for sensitive detection in techniques like Western blotting, ELISA, and immunohistochemistry. The ability to buy and use this reagent simplifies the creation of detection reagents, enhancing experimental sensitivity and specificity. Furthermore, the reagent's property of forming a stable amide bond ensures that the biotinylation is permanent, suitable for various experimental conditions.

Beyond protein labeling, Biotin-NHS Ester finds applications in modifying surfaces for biosensors or affinity chromatography. By functionalizing surfaces with amine groups and then reacting them with Biotin-NHS Ester, researchers can create surfaces with immobilized biotin for capturing biotinylated molecules. This is crucial in developing diagnostic platforms and purification systems. The reagent’s chemical properties, including its white powder appearance and requirement for organic solvents like DMSO or DMF for dissolution, are important considerations for experimental protocols.

As a dedicated supplier, we offer Biotin-NHS Ester with a guaranteed purity of 99% HPLC. This high purity is critical for ensuring the specificity and reliability of bioconjugation reactions, minimizing side reactions and variations in experimental results. We cater to the needs of researchers looking to purchase this essential chemical, offering competitive pricing and flexible quantities. Whether you are engaged in fundamental research or developing diagnostic kits, our Biotin-NHS Ester provides a reliable solution.

We encourage researchers to explore the potential of Biotin-NHS Ester in their work. For detailed protocols, technical specifications, and to request a quote for purchase, please reach out to us. Our commitment is to provide the scientific community with high-quality reagents to facilitate innovation in bioconjugation and molecular labeling.