In the intricate landscape of modern biological research, precision and reliability in molecular labeling are paramount. Biotin-NHS Ester stands as a critical reagent, enabling researchers to precisely modify biomolecules. As a dedicated supplier, we are committed to providing scientists with high-purity Biotin-NHS Ester, facilitating advancements across numerous research areas.

Biotin-NHS Ester is an activated ester derivative of biotin, a vitamin essential for various metabolic processes and a powerful tool in molecular biology due to its extremely high affinity for avidin and streptavidin. The NHS ester group on Biotin-NHS Ester is highly reactive towards primary amines, commonly found on lysine residues and the N-termini of proteins and peptides. This reaction forms a stable amide bond, effectively conjugating biotin to the target molecule. The reagent is typically supplied as a white powder and is soluble in organic solvents such as DMSO and DMF, necessitating proper handling and preparation for use in aqueous buffer systems.

The applications of Biotin-NHS Ester are broad and impactful. In protein labeling, it allows for the modification of antibodies, enzymes, and other proteins, which can then be detected with high sensitivity using avidin or streptavidin conjugates. This is fundamental for techniques like ELISA, Western blotting, and immunocytochemistry, where enhanced detection is crucial. Furthermore, it plays a vital role in creating biotinylated probes for various molecular detection assays, including nucleic acid detection and cell surface labeling. Researchers frequently seek to buy this reagent for its versatility in creating research tools.

The efficacy of any bioconjugation strategy hinges on the quality of the reagents used. Our company specializes in supplying Biotin-NHS Ester with a purity of 99% HPLC. This high standard ensures that researchers receive a reagent that performs consistently, minimizing background noise and maximizing signal specificity. For those looking to purchase reagents, especially in larger quantities for ongoing research or development projects, securing a reliable supplier is key. We offer competitive pricing and support for bulk inquiries, ensuring that essential research materials are accessible.

Understanding the chemical properties and optimal usage conditions for Biotin-NHS Ester is vital for successful implementation. Its reactivity with amines, solubility characteristics, and storage requirements (-20°C) are critical factors for researchers to consider. As a manufacturer and supplier, we aim to provide not just a product, but also the assurance of quality and reliability. We invite you to contact us for a quote and to learn more about how our high-purity Biotin-NHS Ester can support your groundbreaking research in molecular labeling and bioconjugation.