The selection of an appropriate bioconjugation linker is a critical decision that can significantly impact the success of a research project or the development of a therapeutic. Linkers serve as molecular bridges, connecting different biomolecules or functionalizing surfaces. Among the vast array of available crosslinking agents, N-Succinimidyl 11-(maleimido)undecanoate (CAS 87981-04-2) offers a compelling combination of specificity, versatility, and reliability that makes it a preferred choice for many applications.

At the heart of N-Succinimidyl 11-(maleimido)undecanoate's utility lies its bifunctional nature. It features two distinct reactive groups: a maleimide and an N-hydroxysuccinimide (NHS) ester. This dual functionality allows researchers to perform sequential or selective conjugation strategies, crucial for building complex molecular constructs such as Antibody-Drug Conjugates (ADCs), antibody fragments, or antibody-peptide conjugates.

The maleimide end of the linker exhibits high specificity for thiol (sulfhydryl) groups. Thiols are primarily found in cysteine residues of proteins and peptides. The reaction between the maleimide and a thiol is rapid and occurs under physiological conditions, forming a stable covalent thioether bond. This specificity is advantageous as it minimizes non-specific reactions with other amino acid side chains, leading to cleaner and more controlled conjugations. For researchers requiring precise thiol conjugation, finding a reliable supplier of N-Succinimidyl 11-(maleimido)undecanoate is essential.

The NHS ester functionality offers complementary reactivity, targeting primary amines. These are present in lysine residues, N-termini of proteins and peptides, and can also be introduced through chemical modification of other molecules. The reaction with amines forms a stable amide bond. This allows N-Succinimidyl 11-(maleimido)undecanoate to be used in a broader range of applications where amine-containing molecules are involved, such as conjugating small molecule drugs, labeling nucleic acids, or attaching molecules to surfaces functionalized with amines.

The ten-carbon aliphatic spacer arm separating the maleimide and NHS ester groups is another key feature. This flexible chain provides adequate distance between the conjugated molecules, which can be crucial for maintaining the activity and proper orientation of the biomolecules involved. For instance, in ADCs, sufficient spacing can ensure that the antibody's binding site remains unobstructed, while the drug payload is effectively released upon reaching the target cell.

When considering the price of N-Succinimidyl 11-(maleimido)undecanoate, researchers often weigh it against the performance benefits. Sourcing from reputable manufacturers in China ensures not only competitive pricing but also access to high-purity material that is critical for reproducible results. Many organizations actively seek to buy N-Succinimidyl 11-(maleimido)undecanoate from trusted vendors who can provide lot-specific Certificates of Analysis (CoA) and robust technical support.

The applications of this linker are vast, spanning drug discovery, diagnostics, and advanced materials science. It is instrumental in creating:

  • Antibody-Drug Conjugates (ADCs) for targeted cancer therapy.
  • Antibody fragments or antibody conjugates for improved pharmacokinetics or diagnostic imaging.
  • Immobilized enzymes or antibodies for biosensors and diagnostic platforms.
  • Peptide conjugates for vaccine development or therapeutic applications.
  • Functionalized nanoparticles for drug delivery.

In summary, N-Succinimidyl 11-(maleimido)undecanoate represents a powerful and versatile tool for bioconjugation. Its specific thiol-maleimide and amine-NHS ester chemistries, combined with an optimal linker arm, make it ideal for a wide array of applications in life sciences. Ensuring a reliable supply of this high-quality reagent from experienced manufacturers is a critical step for any research team aiming for precision and success.