The ability to visualize biological processes within living organisms is crucial for understanding health and disease. In vivo bioluminescence imaging (BLI) offers a highly sensitive method for this purpose, and Coelenterazine, a key bioluminescent substrate with CAS number 55779-48-1, is central to its application, especially for deep tissue studies.

Coelenterazine acts as the light-generating substrate for various luciferases, such as Renilla luciferase (RLuc), which are commonly used in BLI systems. When these enzymes are expressed in cells or tissues and provided with Coelenterazine, they catalyze an oxidation reaction that emits light. This emitted light can then be detected by specialized cameras, allowing researchers to track biological events non-invasively within a living animal.

A significant advantage of bioluminescence using Coelenterazine is its inherent low background signal. Unlike fluorescence imaging, which requires an external excitation light source that can cause autofluorescence and scatter, bioluminescence generates its own light. This makes it particularly effective for detecting weak signals from deep within tissues, where excitation light penetration is limited. For researchers focused on in vivo applications, sourcing high-quality Coelenterazine is essential for obtaining clear and interpretable data. NINGBO INNO PHARMCHEM CO.,LTD. understands these demands and is a reliable supplier in China.

Coelenterazine plays a vital role in various aspects of in vivo research, including tracking tumor growth and metastasis, monitoring gene therapy delivery, and evaluating drug efficacy and distribution. By attaching luciferase genes to specific cell types or disease markers, researchers can visualize their location and activity through the light produced by the Coelenterazine-mediated reaction. This capability significantly accelerates preclinical studies and drug development pipelines.

The development of Coelenterazine analogues also contributes to advancing in vivo BLI. Modified Coelenterazine molecules can be designed to emit light at longer wavelengths, which penetrate tissues more effectively, or to have improved stability and kinetic profiles. These chemical innovations are vital for expanding the scope and sensitivity of deep-tissue imaging. The consistent supply of Coelenterazine and its derivatives is a critical factor for researchers looking to buy these advanced tools.

In conclusion, Coelenterazine (CAS: 55779-48-1) is a fundamental substrate enabling powerful in vivo bioluminescence imaging. Its capacity to generate light signals with low background makes it ideal for deep tissue studies and drug development. NINGBO INNO PHARMCHEM CO.,LTD. is committed to supporting scientific progress by providing high-purity Coelenterazine. If you are seeking to purchase Coelenterazine for your in vivo research, our quality and reliability will ensure your experimental success.