In the realm of biological research, understanding cellular mechanisms and signaling pathways is paramount. Bioluminescence, the production of light by living organisms, offers a powerful non-invasive tool for such investigations. At the heart of many bioluminescent systems lies Coelenterazine, a fascinating molecule with the CAS number 55779-48-1.

Coelenterazine serves as the essential substrate for a variety of luciferases and photoproteins, most notably those found in marine organisms like Renilla reniformis and Aequorea victoria. When these enzymes catalyze the oxidation of Coelenterazine, light is emitted, a process that can be harnessed for a multitude of scientific applications. The efficiency and sensitivity of this light emission make Coelenterazine an indispensable reagent for many laboratories.

One of the most significant applications of Coelenterazine is in the measurement of intracellular calcium ion concentrations. Photoproteins such as aequorin, when bound to calcium, undergo a conformational change that leads to the oxidation of Coelenterazine and the subsequent emission of light. This allows researchers to monitor real-time changes in calcium levels within living cells, providing critical insights into processes like muscle contraction, neurotransmitter release, and cell signaling. For those looking to purchase Coelenterazine for these demanding assays, sourcing high-quality material is crucial.

Furthermore, Coelenterazine is widely employed as a substrate for Renilla luciferase (RLuc) in reporter gene assays. These assays are invaluable for studying gene expression, protein-protein interactions, and the efficacy of drug candidates. By linking the expression of RLuc to a specific gene or promoter, scientists can quantify the activity of that gene or promoter by measuring the light output generated from Coelenterazine. The reliability of the Coelenterazine supply directly impacts the reproducibility of these experiments.

The chemical synthesis of Coelenterazine and its derivatives has also been an area of intense research. Modifications to the Coelenterazine structure can lead to analogues with altered spectral properties, such as shifted emission wavelengths, or improved stability and kinetic properties. These novel analogues expand the toolkit available for bioluminescent imaging, enabling multicolor imaging and applications in deeper tissues. Researchers often seek reliable suppliers who can provide not only the native Coelenterazine but also these advanced derivatives for specific experimental needs.

As a leading manufacturer and supplier in China, NINGBO INNO PHARMCHEM CO.,LTD. is dedicated to providing researchers with premium Coelenterazine and its related products. Our commitment to quality ensures that your experiments relying on Coelenterazine for bioluminescence imaging or calcium measurements will yield accurate and reproducible results. We understand the critical role this molecule plays and strive to be your trusted partner in scientific discovery. If you are looking to buy Coelenterazine, consider the quality and consistency we offer to drive your research forward.