In the dynamic field of life sciences, tools that enable precise and sensitive observation of biological phenomena are invaluable. Coelenterazine, a bioluminescent substrate with CAS number 55779-48-1, stands out as one such versatile molecule. Its ability to produce light in enzymatic reactions has made it a cornerstone for various research applications, from understanding cellular signaling to advancing imaging technologies.

Coelenterazine is the essential substrate for a family of enzymes known as luciferases, particularly those found in marine organisms. When these enzymes, such as Renilla luciferase (RLuc) or photoproteins like aequorin, catalyze the oxidation of Coelenterazine, a light-emitting reaction occurs. This process forms the basis of bioluminescent assays, which are favored for their high sensitivity and low background signal, making them ideal for detecting even minute biological activities.

One of Coelenterazine's most critical applications is in the measurement of intracellular calcium ions. When calcium ions bind to photoproteins like aequorin, they trigger a cascade that involves the oxidation of Coelenterazine. The resulting light emission is directly correlated with the calcium concentration, allowing researchers to monitor dynamic changes in cellular calcium levels, which are fundamental to numerous physiological processes. For scientists requiring reliable calcium measurements, purchasing high-purity Coelenterazine is essential.

Furthermore, Coelenterazine is a key component in bioluminescent reporter gene systems, predominantly used with Renilla luciferase. These systems are widely employed to study gene expression, promoter activity, and the interactions of proteins. By quantifying the light produced from Coelenterazine-mediated reactions, researchers can gain quantitative insights into gene regulation and cellular responses to various stimuli. The accessibility and cost-effectiveness of Coelenterazine contribute to its widespread use in these applications, making it a popular choice for researchers looking to buy these essential reagents.

The chemical structure of Coelenterazine also lends itself to modification, leading to the development of various analogues. These derivatives can offer enhanced properties, such as improved brightness, altered emission wavelengths for multiplexing, or increased stability. These advancements are crucial for developing next-generation bioluminescent imaging tools that can probe complex biological questions. NINGBO INNO PHARMCHEM CO.,LTD., as a leading manufacturer in China, is involved in the chemical synthesis that makes these vital compounds available.

In essence, Coelenterazine (CAS: 55779-48-1) is a versatile and indispensable molecule for modern scientific discovery. Its role in bioluminescence, particularly in calcium sensing and reporter gene assays, underscores its importance. NINGBO INNO PHARMCHEM CO.,LTD. is committed to providing researchers with premium Coelenterazine, ensuring the quality and reliability needed for groundbreaking scientific work. If you need to purchase Coelenterazine, our dedication to excellence guarantees satisfaction.