Reporter gene assays are fundamental tools in molecular biology, allowing scientists to quantify gene expression, promoter activity, and cellular responses to stimuli. Among the various reporter systems, those utilizing bioluminescence, specifically employing Renilla luciferase (RLuc) and its substrate Coelenterazine, are highly favored for their sensitivity and broad dynamic range. Coelenterazine, with its CAS number 55779-48-1, is the critical component that fuels this powerful detection system.

Renilla luciferase, derived from the sea pansy *Renilla reniformis*, catalyzes the oxidation of Coelenterazine, producing a light signal that is directly proportional to the amount of luciferase present. This makes it an ideal reporter enzyme. When researchers want to study the regulation of a specific gene or the strength of a promoter, they can fuse the gene or promoter sequence to the Renilla luciferase gene. Subsequently, the expression level of the reporter gene can be quantified by measuring the bioluminescent output after adding Coelenterazine. This allows for precise monitoring of transcriptional activity.

The sensitivity of these assays is highly dependent on the quality and availability of Coelenterazine. As a dedicated supplier in China, NINGBO INNO PHARMCHEM CO.,LTD. ensures that the Coelenterazine we provide meets stringent purity standards, guaranteeing consistent and reliable light emission. This consistency is vital for quantitative analysis and for comparing results across different experiments or laboratories. Researchers often look for competitive pricing and dependable supply when they need to buy Coelenterazine in bulk for high-throughput screening or extensive research projects.

Beyond basic gene expression studies, Coelenterazine-based RLuc assays are widely used in drug discovery and development. Pharmaceutical companies employ these assays to screen large libraries of compounds for their ability to modulate gene expression or cellular pathways. The bioluminescent readout allows for rapid and sensitive detection of active compounds, significantly accelerating the drug discovery process. The ability to easily purchase Coelenterazine from a trusted source like NINGBO INNO PHARMCHEM CO.,LTD. is critical for the success of these large-scale screening campaigns.

The chemical structure of Coelenterazine also allows for the development of various analogues. These modified Coelenterazine molecules can exhibit different kinetic properties, stability, or emission spectra, providing further flexibility in assay design. For instance, some analogues might offer brighter signals or longer emission kinetics, which can be advantageous for specific experimental setups. Understanding the chemical synthesis of these compounds is key to their development, and NINGBO INNO PHARMCHEM CO.,LTD. stays at the forefront of providing these advanced reagents.

In summary, Coelenterazine (CAS: 55779-48-1) is not just a chemical compound; it is a cornerstone of modern molecular biology research, enabling sensitive and quantitative analysis through bioluminescence. Whether you are studying gene regulation, screening potential therapeutics, or investigating cellular signaling, high-quality Coelenterazine is essential. NINGBO INNO PHARMCHEM CO.,LTD. is committed to supporting your research by being a reliable manufacturer and supplier, ensuring you can buy the best Coelenterazine for your needs.