The Critical Role of Fmoc-Ser(tBu)-OH in Fmoc Peptide Synthesis
The advancements in peptide synthesis have revolutionized fields ranging from medicine to materials science. Central to these advancements is the development and availability of high-quality protected amino acid derivatives, such as Fmoc-Ser(tBu)-OH. This compound is a cornerstone for researchers employing the Fmoc strategy in solid-phase peptide synthesis (SPPS), ensuring the successful construction of complex and biologically active peptides.
Fmoc-Ser(tBu)-OH, chemically named Nα-Fmoc-O-tert-butyl-L-serine, is a derivative of the amino acid serine. Its defining features are the Fmoc (9-fluorenylmethoxycarbonyl) group attached to the alpha-amino group and the tert-butyl (tBu) ether protecting group on the side-chain hydroxyl. The Fmoc group offers a base-labile protection, allowing for facile deprotection with reagents like piperidine, which is central to the iterative SPPS cycle. The tert-butyl group provides robust protection for the serine side chain throughout the synthesis, preventing unwanted side reactions such as alkylation or esterification during coupling and deprotection steps.
When researchers decide to buy Fmoc-Ser(tBu)-OH, the purity of the material is a critical consideration. High-purity grades, often exceeding 99%, are essential for achieving high yields and minimizing impurities in the final peptide product. The CAS number 71989-33-8 is the standard identifier for this compound, aiding in its procurement. Sourcing this reagent from reputable manufacturers and suppliers ensures that the product meets the stringent quality standards required for advanced peptide synthesis applications.
The utility of Fmoc-Ser(tBu)-OH is vast, primarily in the field of custom peptide manufacturing. It allows for the precise incorporation of serine residues, which are common in many natural peptides and proteins and often play roles in structural stability or post-translational modifications. The tert-butyl protecting group is strategically chosen because it can be efficiently removed along with other acid-labile protecting groups during the final cleavage of the peptide from the resin, typically using trifluoroacetic acid (TFA) cocktails.
For laboratories dedicated to peptide research and development, securing a consistent and reliable supply of Fmoc-Ser(tBu)-OH is vital. Companies like NINGBO INNO PHARMCHEM CO.,LTD., a manufacturer based in China, are key players in providing these essential chemical building blocks to the global scientific community. Their commitment to quality ensures that researchers have access to the high-purity reagents necessary for groundbreaking discoveries in peptide chemistry and beyond.
Fmoc-Ser(tBu)-OH, chemically named Nα-Fmoc-O-tert-butyl-L-serine, is a derivative of the amino acid serine. Its defining features are the Fmoc (9-fluorenylmethoxycarbonyl) group attached to the alpha-amino group and the tert-butyl (tBu) ether protecting group on the side-chain hydroxyl. The Fmoc group offers a base-labile protection, allowing for facile deprotection with reagents like piperidine, which is central to the iterative SPPS cycle. The tert-butyl group provides robust protection for the serine side chain throughout the synthesis, preventing unwanted side reactions such as alkylation or esterification during coupling and deprotection steps.
When researchers decide to buy Fmoc-Ser(tBu)-OH, the purity of the material is a critical consideration. High-purity grades, often exceeding 99%, are essential for achieving high yields and minimizing impurities in the final peptide product. The CAS number 71989-33-8 is the standard identifier for this compound, aiding in its procurement. Sourcing this reagent from reputable manufacturers and suppliers ensures that the product meets the stringent quality standards required for advanced peptide synthesis applications.
The utility of Fmoc-Ser(tBu)-OH is vast, primarily in the field of custom peptide manufacturing. It allows for the precise incorporation of serine residues, which are common in many natural peptides and proteins and often play roles in structural stability or post-translational modifications. The tert-butyl protecting group is strategically chosen because it can be efficiently removed along with other acid-labile protecting groups during the final cleavage of the peptide from the resin, typically using trifluoroacetic acid (TFA) cocktails.
For laboratories dedicated to peptide research and development, securing a consistent and reliable supply of Fmoc-Ser(tBu)-OH is vital. Companies like NINGBO INNO PHARMCHEM CO.,LTD., a manufacturer based in China, are key players in providing these essential chemical building blocks to the global scientific community. Their commitment to quality ensures that researchers have access to the high-purity reagents necessary for groundbreaking discoveries in peptide chemistry and beyond.
Perspectives & Insights
Bio Analyst 88
“The tert-butyl group provides robust protection for the serine side chain throughout the synthesis, preventing unwanted side reactions such as alkylation or esterification during coupling and deprotection steps.”
Nano Seeker Pro
“When researchers decide to buy Fmoc-Ser(tBu)-OH, the purity of the material is a critical consideration.”
Data Reader 7
“High-purity grades, often exceeding 99%, are essential for achieving high yields and minimizing impurities in the final peptide product.”