Choosing the Right Fmoc Amino Acid: Focus on Fmoc-Ser(tBu)-OH
The success of peptide synthesis hinges significantly on the quality and appropriate selection of the protected amino acid derivatives used. Among these, Fmoc-Ser(tBu)-OH holds a prominent position, serving as a critical component for introducing serine residues into peptide chains via the widely used Fmoc (9-fluorenylmethoxycarbonyl) solid-phase peptide synthesis (SPPS) strategy.
Fmoc-Ser(tBu)-OH, scientifically identified as Nα-Fmoc-O-tert-butyl-L-serine, is engineered with specific protecting groups to facilitate controlled peptide chain elongation. The Fmoc group on the alpha-amino terminus is base-labile, allowing for its removal under mild conditions in each cycle of SPPS. Crucially, the tert-butyl (tBu) ether group protects the hydroxyl side chain of serine. This side-chain protection is essential as it prevents unintended reactions, such as lactone formation or unwanted esterification, during the coupling of amino acids and subsequent deprotection steps.
For researchers and chemists aiming to buy Fmoc-Ser(tBu)-OH, prioritizing high purity is paramount. Material with purity levels exceeding 99% is typically required to ensure the efficiency of coupling reactions and the overall quality of the final peptide product. The CAS number 71989-33-8 is the universally recognized identifier for this compound, making it easier to source. Reputable suppliers provide detailed specifications and Certificates of Analysis (CoA) to verify the purity and identity of the reagent.
The applications for Fmoc-Ser(tBu)-OH are diverse, primarily within the realm of custom peptide manufacturing and the development of peptide-based pharmaceuticals. Serine residues are frequently found in biologically active peptides, often participating in hydrogen bonding or serving as sites for post-translational modifications. The tert-butyl protecting group is favored for its stability during synthesis and its efficient removal under acidic conditions, which are typically employed during the final cleavage of the peptide from the solid support.
Selecting the right Fmoc amino acid, such as high-quality Fmoc-Ser(tBu)-OH, is a critical step for any peptide synthesis project. Ensuring a consistent supply of this reagent from trusted manufacturers, like NINGBO INNO PHARMCHEM CO.,LTD., empowers researchers to achieve accurate and reproducible results, driving progress in peptide chemistry and its myriad applications.
Fmoc-Ser(tBu)-OH, scientifically identified as Nα-Fmoc-O-tert-butyl-L-serine, is engineered with specific protecting groups to facilitate controlled peptide chain elongation. The Fmoc group on the alpha-amino terminus is base-labile, allowing for its removal under mild conditions in each cycle of SPPS. Crucially, the tert-butyl (tBu) ether group protects the hydroxyl side chain of serine. This side-chain protection is essential as it prevents unintended reactions, such as lactone formation or unwanted esterification, during the coupling of amino acids and subsequent deprotection steps.
For researchers and chemists aiming to buy Fmoc-Ser(tBu)-OH, prioritizing high purity is paramount. Material with purity levels exceeding 99% is typically required to ensure the efficiency of coupling reactions and the overall quality of the final peptide product. The CAS number 71989-33-8 is the universally recognized identifier for this compound, making it easier to source. Reputable suppliers provide detailed specifications and Certificates of Analysis (CoA) to verify the purity and identity of the reagent.
The applications for Fmoc-Ser(tBu)-OH are diverse, primarily within the realm of custom peptide manufacturing and the development of peptide-based pharmaceuticals. Serine residues are frequently found in biologically active peptides, often participating in hydrogen bonding or serving as sites for post-translational modifications. The tert-butyl protecting group is favored for its stability during synthesis and its efficient removal under acidic conditions, which are typically employed during the final cleavage of the peptide from the solid support.
Selecting the right Fmoc amino acid, such as high-quality Fmoc-Ser(tBu)-OH, is a critical step for any peptide synthesis project. Ensuring a consistent supply of this reagent from trusted manufacturers, like NINGBO INNO PHARMCHEM CO.,LTD., empowers researchers to achieve accurate and reproducible results, driving progress in peptide chemistry and its myriad applications.
Perspectives & Insights
Data Seeker X
“, empowers researchers to achieve accurate and reproducible results, driving progress in peptide chemistry and its myriad applications.”
Chem Reader AI
“The success of peptide synthesis hinges significantly on the quality and appropriate selection of the protected amino acid derivatives used.”
Agile Vision 2025
“Among these, Fmoc-Ser(tBu)-OH holds a prominent position, serving as a critical component for introducing serine residues into peptide chains via the widely used Fmoc (9-fluorenylmethoxycarbonyl) solid-phase peptide synthesis (SPPS) strategy.”