Understanding 2-Mercaptoethanol: Properties and Key Uses for Researchers
2-Mercaptoethanol, also known as 2-hydroxy-1-ethanethiol or beta-mercaptoethanol, is a bifunctional molecule featuring both a hydroxyl (-OH) and a thiol (-SH) group. This unique structure grants it a distinct chemical reactivity, making it a valuable tool for researchers across various scientific disciplines. Its low molecular weight (78.13 g/mol) and solubility in water and common organic solvents further enhance its utility. NINGBO INNO PHARMCHEM CO.,LTD. offers this vital compound for your research endeavors.
The most prominent chemical property of 2-Mercaptoethanol is its potent reducing capability, primarily attributed to the thiol group. This allows it to readily donate hydrogen atoms, effectively reducing disulfide bonds (-S-S-) in proteins to their constituent sulfhydryl groups (-SH). This reaction is fundamental in biochemical research for processes such as protein denaturation, maintaining protein conformation, and preventing unwanted oxidation during sample handling.
In protein biochemistry, researchers frequently use 2-Mercaptoethanol in conjunction with denaturants like urea or guanidine hydrochloride to ensure complete unfolding of polypeptide chains. This is particularly important for techniques like SDS-PAGE, where accurate molecular weight determination is key. The ability of 2-Mercaptoethanol to break disulfide bonds in keratin and other structural proteins also makes it valuable in cosmetic applications like hair perms and straightening treatments.
Beyond protein studies, 2-Mercaptoethanol serves as a critical component in cell culture media. It acts as an antioxidant, protecting cells from damage caused by reactive oxygen species (ROS) that can be generated during metabolic processes or by external stimuli. This protective role is vital for maintaining cell viability and promoting healthy cell growth in research environments.
For scientists looking to purchase 2-Mercaptoethanol, NINGBO INNO PHARMCHEM CO.,LTD. provides a reliable source of high-purity material. Our dedication to quality ensures that researchers can depend on our product for reproducible and accurate experimental outcomes. Trust NINGBO INNO PHARMCHEM CO.,LTD. to supply the essential reagents that drive scientific discovery.
The most prominent chemical property of 2-Mercaptoethanol is its potent reducing capability, primarily attributed to the thiol group. This allows it to readily donate hydrogen atoms, effectively reducing disulfide bonds (-S-S-) in proteins to their constituent sulfhydryl groups (-SH). This reaction is fundamental in biochemical research for processes such as protein denaturation, maintaining protein conformation, and preventing unwanted oxidation during sample handling.
In protein biochemistry, researchers frequently use 2-Mercaptoethanol in conjunction with denaturants like urea or guanidine hydrochloride to ensure complete unfolding of polypeptide chains. This is particularly important for techniques like SDS-PAGE, where accurate molecular weight determination is key. The ability of 2-Mercaptoethanol to break disulfide bonds in keratin and other structural proteins also makes it valuable in cosmetic applications like hair perms and straightening treatments.
Beyond protein studies, 2-Mercaptoethanol serves as a critical component in cell culture media. It acts as an antioxidant, protecting cells from damage caused by reactive oxygen species (ROS) that can be generated during metabolic processes or by external stimuli. This protective role is vital for maintaining cell viability and promoting healthy cell growth in research environments.
For scientists looking to purchase 2-Mercaptoethanol, NINGBO INNO PHARMCHEM CO.,LTD. provides a reliable source of high-purity material. Our dedication to quality ensures that researchers can depend on our product for reproducible and accurate experimental outcomes. Trust NINGBO INNO PHARMCHEM CO.,LTD. to supply the essential reagents that drive scientific discovery.
Perspectives & Insights
Nano Explorer 01
“In protein biochemistry, researchers frequently use 2-Mercaptoethanol in conjunction with denaturants like urea or guanidine hydrochloride to ensure complete unfolding of polypeptide chains.”
Data Catalyst One
“This is particularly important for techniques like SDS-PAGE, where accurate molecular weight determination is key.”
Chem Thinker Labs
“The ability of 2-Mercaptoethanol to break disulfide bonds in keratin and other structural proteins also makes it valuable in cosmetic applications like hair perms and straightening treatments.”