Molecular biology research thrives on precision and sensitivity, and the reagents used are critical to achieving reliable results. Among these indispensable tools is 5-Bromo-4-chloro-3-indolyl Phosphate Disodium Salt (BCIP, CAS 102185-33-1), a widely utilized chromogenic substrate for detecting alkaline phosphatase (AP) activity. As a dedicated manufacturer and supplier of high-quality biochemicals, we are proud to provide researchers with BCIP that fuels discovery in this dynamic field.

Alkaline phosphatase is frequently employed as a reporter enzyme in various molecular biology techniques due to its stability and high catalytic efficiency. When conjugated to antibodies, DNA probes, or other biomolecules, AP's presence can be readily detected using substrates like BCIP. Upon enzymatic cleavage by AP, BCIP liberates an indolyl derivative that, especially when paired with a coupling agent like Nitroblue Tetrazolium (NBT), produces a vividly colored, insoluble precipitate. This makes it an excellent tool for researchers looking to buy BCIP for sensitive and localized detection.

Key Molecular Biology Applications of BCIP:

  • Western Blotting: BCIP-NBT is a classic substrate for detecting AP-labeled antibodies, visualizing specific proteins separated by gel electrophoresis. The resulting dark precipitate is highly visible on membranes.
  • Southern and Northern Blotting: Used to detect specific DNA or RNA sequences hybridized to AP-labeled probes, allowing for the analysis of gene expression and DNA structure.
  • ELISA Assays: In enzyme-linked immunosorbent assays, BCIP contributes to sensitive colorimetric detection of antigen-antibody interactions.
  • In Situ Hybridization (ISH): BCIP aids in visualizing the precise location of specific nucleic acid sequences within cells or tissue sections.

Why Procure BCIP from a Trusted Supplier?

The effectiveness of BCIP in these applications directly correlates with its purity and stability. As a reputable 5-bromo-4-chloro-3-indolyl phosphate disodium salt supplier, we ensure that our product meets rigorous standards. This means:

  • Consistent Sensitivity: Our high-purity BCIP provides reliable and sensitive detection, crucial for identifying low-abundance targets.
  • Clear Localization: The insoluble nature of the precipitate ensures that the signal remains localized to the site of enzyme activity, providing clear spatial information.
  • Batch-to-Batch Reproducibility: Our manufacturing processes guarantee consistent quality, so your experiments yield predictable results every time.

For research laboratories requiring a dependable source for CAS 102185-33-1, partnering with a knowledgeable biochemical reagent supplier like us is essential. We encourage you to inquire about our product and pricing to purchase BCIP and enhance your molecular biology research.