In the realm of biochemistry and molecular biology, precisely linking molecules together is often essential for research, diagnostics, and therapeutics. Disuccinimidyl Glutarate (DSG) is a widely used chemical reagent that facilitates such linkages. This article aims to demystify DSG, explaining its properties, applications, and why sourcing it from a reliable manufacturer is crucial for researchers. As a leading biochemical supplier, we offer high-quality DSG to meet your laboratory needs.

Understanding Disuccinimidyl Glutarate (DSG)

Disuccinimidyl Glutarate, also known by its CAS number 79642-50-5, is classified as a homobifunctional crosslinking agent. This means it possesses two identical reactive groups on its molecular structure. In the case of DSG, these are N-hydroxysuccinimide (NHS) esters, located at both ends of a five-carbon (glutarate) chain. These NHS ester groups are highly reactive towards primary amines, making DSG an excellent tool for creating covalent bonds with molecules containing amine functionalities, such as proteins and peptides.

The key characteristics of DSG include:

  • Reactivity: NHS esters readily react with primary amines at physiological pH (7-9), forming stable amide bonds. This specificity ensures targeted crosslinking.
  • Spacer Arm: The five-carbon linker provides a defined distance between the crosslinked molecules, which can be important for maintaining biological activity and preventing steric hindrance.
  • Solubility: DSG is typically soluble in polar organic solvents like dimethyl sulfoxide (DMSO) and dimethylformamide (DMF), facilitating its use in aqueous reaction mixtures.
  • Membrane Permeability: Notably, DSG can permeate cell membranes, making it suitable for intracellular crosslinking applications.

Applications of DSG in Scientific Research:

The versatility of DSG makes it valuable across various scientific disciplines:

  • Protein Modification: DSG is frequently used to link proteins to other molecules, such as labeling proteins with fluorescent tags or immobilizing enzymes onto surfaces. Researchers often buy DSG for its reliable performance in these modifications.
  • Studying Protein Interactions: By stabilizing protein complexes, DSG aids in the identification and characterization of protein-protein interactions, which is critical for understanding cellular pathways.
  • Chromatin Immunoprecipitation (ChIP): DSG helps to fix protein-DNA complexes, enhancing the accuracy of ChIP assays used to map protein binding sites on genomic DNA.
  • Antibody-Drug Conjugates (ADCs): In pharmaceutical research, DSG can serve as a linker for creating ADCs, a class of targeted cancer therapies.

Why Choose a High-Quality DSG Supplier?

The effectiveness of any chemical reagent, including DSG, depends heavily on its quality. Sourcing Disuccinimidyl Glutarate from a reputable manufacturer or supplier is essential for several reasons:

  • Purity: High purity (often exceeding 97%) ensures specific reactivity and minimizes unwanted side reactions, leading to more accurate and reproducible results.
  • Consistency: Reliable manufacturers provide consistent batch-to-batch quality, which is crucial for maintaining experimental integrity over time.
  • Technical Support: Reputable suppliers often offer technical assistance and product documentation, aiding researchers in optimizing their experimental protocols.

We are committed to being a trusted source for your biochemical needs. Our Disuccinimidyl Glutarate is manufactured under stringent quality control to ensure high purity and consistent performance. Whether you are initiating a new research project or scaling up production, we are equipped to supply the quality DSG you require. If you need to purchase DSG, look no further than our reliable offerings.

To ensure the success of your experiments, always opt for high-quality Disuccinimidyl Glutarate from a trusted partner. Contact us today to discuss your requirements, get a quote, or place an order for DSG.