For professionals in the pharmaceutical and biotech sectors, the efficient and accurate synthesis of peptides is paramount. Central to this process, especially within the widely adopted Fmoc (9-fluorenylmethyloxycarbonyl) solid-phase peptide synthesis (SPPS) methodology, is the use of protected amino acid derivatives. Among these, Fmoc-L-Arg(Pbf)-OH, identified by CAS number 154445-77-9, stands out as a critical building block.

This article aims to provide a comprehensive overview of Fmoc-L-Arg(Pbf)-OH from a supplier's perspective, detailing its significance, properties, and the advantages it offers to researchers and manufacturers. Understanding its role can help you make informed purchasing decisions and optimize your peptide synthesis workflows. If you are looking to buy Fmoc-L-Arg(Pbf)-OH, understanding its benefits is key to securing a high-quality product from a reliable manufacturer in China.

The Importance of Protected Arginine

Arginine is a fundamental amino acid characterized by its positively charged guanidino group. This functional group is highly reactive and can participate in various side reactions during peptide synthesis, such as alkylation or aggregation, leading to lower yields and product impurities. To circumvent these issues, protective groups are essential.

Fmoc-L-Arg(Pbf)-OH is specifically designed to address this challenge. It features two key protective elements:

  • Fmoc Group: Attached to the alpha-amino group, the Fmoc group is base-labile, meaning it can be easily removed by reagents like piperidine. This deprotection step is crucial for allowing the next amino acid to couple to the growing peptide chain.
  • Pbf Group (2,2,4,6,7-pentamethyldihydrobenzofuran-5-sulfonyl): This group protects the guanidino side chain of arginine. The Pbf group is acid-labile, designed to be removed during the final cleavage step of SPPS, typically using trifluoroacetic acid (TFA) cocktails. Its selection offers a good balance between stability during synthesis and clean removal at the end.

Key Properties and Advantages

As a reputable supplier of Fmoc-Arg(Pbf)-OH, we emphasize the following properties that make this compound indispensable:

  • High Purity: Our Fmoc-L-Arg(Pbf)-OH is manufactured to stringent purity standards (typically ≥99.0%), ensuring predictable and reproducible results in your peptide synthesis. This is vital for pharmaceutical intermediates and research applications where impurities can compromise outcomes.
  • Solubility: It exhibits good solubility in common SPPS solvents like Dimethylformamide (DMF) and Dimethyl Sulfoxide (DMSO), facilitating efficient reactions.
  • Stability: The molecule remains stable under typical Fmoc SPPS conditions, preventing premature deprotection of either the Fmoc or Pbf groups until desired.
  • Yield Improvement: By effectively masking the reactive guanidine group, Fmoc-L-Arg(Pbf)-OH significantly reduces side reactions, leading to higher overall yields of your target peptides.
  • Compatibility: It is fully compatible with automated peptide synthesizers and standard Fmoc SPPS protocols, making it an easy addition to your existing processes.

Applications in Peptide Synthesis

The primary application of Fmoc-L-Arg(Pbf)-OH is in the synthesis of peptides via Fmoc SPPS. This includes:

  • Therapeutic Peptides: Arginine-rich peptides are prevalent in many therapeutic areas, including cell-penetrating peptides (CPPs), antimicrobial peptides, and peptide hormones. The correct incorporation of arginine using Fmoc-L-Arg(Pbf)-OH is critical for their efficacy and safety.
  • Research and Development: Academic and industrial researchers rely on high-purity Fmoc-Arg(Pbf)-OH for synthesizing peptides for drug discovery, structural biology, and biochemical assays. Purchasing from a reliable source ensures the integrity of your research.
  • Bioconjugation: It can also be used in creating peptide conjugates for diagnostic or therapeutic applications, where the arginine residue might play a role in targeting or solubility.

Why Choose Our Fmoc-L-Arg(Pbf)-OH?

When seeking to purchase Fmoc-Arg(Pbf)-OH, partnering with a reputable supplier is crucial. We offer:

  • Competitive Pricing: We provide cost-effective solutions for your peptide synthesis needs, with attractive price points for bulk orders.
  • Reliable Supply Chain: As a leading manufacturer based in China, we ensure a stable and consistent supply of this essential reagent.
  • Technical Support: Our team is available to provide guidance on product specifications and applications.

In conclusion, Fmoc-L-Arg(Pbf)-OH is an indispensable tool for peptide chemists. Its ability to efficiently and cleanly incorporate arginine into peptide sequences makes it a vital component for successful peptide synthesis. We invite you to contact us for a quote and to discuss your bulk purchasing requirements for high-quality Fmoc-L-Arg(Pbf)-OH.