The Chemistry Behind BCIP p-Toluidine Salt: A Key Intermediate and Substrate
BCIP p-toluidine salt, also identified by its CAS number 6578-06-9, is a compound of significant interest in both biochemistry and chemical synthesis. Its dual role as a highly effective chromogenic substrate for alkaline phosphatase and as a valuable intermediate in various chemical processes makes it a versatile reagent for a wide array of scientific applications.
As a chromogenic substrate, BCIP p-toluidine salt is integral to detection systems where alkaline phosphatase (AP) activity needs to be visualized. The enzymatic hydrolysis of BCIP p-toluidine salt by AP releases an indolyl derivative. This intermediate undergoes oxidation, typically in the presence of an oxidant like NBT, to form an insoluble, intensely colored precipitate. This reaction is fundamental to techniques like Western blotting, immunohistochemistry, and in situ hybridization, enabling researchers to pinpoint the location and quantify the activity of AP-labeled molecules.
The chemical structure of BCIP p-toluidine salt contributes to its specific properties, including its solubility in solvents such as dimethylformamide (DMF). The high purity, often specified as 99% minimum assay, ensures reliable performance in sensitive detection protocols and chemical synthesis. This compound serves as a crucial pharmaceutical intermediate, playing a role in the multi-step synthesis of various medicinal compounds. Furthermore, its utility extends to the agrochemical industry, where it functions as an intermediate in the creation of specialized agricultural chemicals.
For entities looking to purchase BCIP p-toluidine salt, the Chinese market offers a robust supply of high-quality material. Sourcing from Chinese manufacturers at wholesale prices provides an economically viable option for extensive research or large-scale production. The commitment to quality and consistent assay levels from these suppliers is critical for researchers and industrial chemists alike. Whether used for precise biochemical detection or as a foundational element in complex syntheses, BCIP p-toluidine salt remains an indispensable chemical compound.
Understanding the chemistry and application of BCIP p-toluidine salt empowers scientists and manufacturers to leverage its full potential. Its consistent performance as both a detection tool and a synthetic intermediate underscores its importance in modern chemical and biological research.
As a chromogenic substrate, BCIP p-toluidine salt is integral to detection systems where alkaline phosphatase (AP) activity needs to be visualized. The enzymatic hydrolysis of BCIP p-toluidine salt by AP releases an indolyl derivative. This intermediate undergoes oxidation, typically in the presence of an oxidant like NBT, to form an insoluble, intensely colored precipitate. This reaction is fundamental to techniques like Western blotting, immunohistochemistry, and in situ hybridization, enabling researchers to pinpoint the location and quantify the activity of AP-labeled molecules.
The chemical structure of BCIP p-toluidine salt contributes to its specific properties, including its solubility in solvents such as dimethylformamide (DMF). The high purity, often specified as 99% minimum assay, ensures reliable performance in sensitive detection protocols and chemical synthesis. This compound serves as a crucial pharmaceutical intermediate, playing a role in the multi-step synthesis of various medicinal compounds. Furthermore, its utility extends to the agrochemical industry, where it functions as an intermediate in the creation of specialized agricultural chemicals.
For entities looking to purchase BCIP p-toluidine salt, the Chinese market offers a robust supply of high-quality material. Sourcing from Chinese manufacturers at wholesale prices provides an economically viable option for extensive research or large-scale production. The commitment to quality and consistent assay levels from these suppliers is critical for researchers and industrial chemists alike. Whether used for precise biochemical detection or as a foundational element in complex syntheses, BCIP p-toluidine salt remains an indispensable chemical compound.
Understanding the chemistry and application of BCIP p-toluidine salt empowers scientists and manufacturers to leverage its full potential. Its consistent performance as both a detection tool and a synthetic intermediate underscores its importance in modern chemical and biological research.
Perspectives & Insights
Molecule Vision 7
“Its consistent performance as both a detection tool and a synthetic intermediate underscores its importance in modern chemical and biological research.”
Alpha Origin 24
“BCIP p-toluidine salt, also identified by its CAS number 6578-06-9, is a compound of significant interest in both biochemistry and chemical synthesis.”
Future Analyst X
“Its dual role as a highly effective chromogenic substrate for alkaline phosphatase and as a valuable intermediate in various chemical processes makes it a versatile reagent for a wide array of scientific applications.”