Fmoc-Thr(tBu)-OL: Bulk Supply & Technical Specs for Peptide Synthesis
Technical Specifications and Purity Grades of Fmoc-Thr(tBu)-OL
Fmoc-Thr(tBu)-OL, also known as Fmoc-Threoninol or 9H-Fluoren-9-ylmethyl carbamate, is a critical chemical building block in solid-phase peptide synthesis (SPPS). As a protected amino alcohol, it serves as a versatile intermediate for constructing peptide alcohols and modified peptides. At NINGBO INNO PHARMCHEM CO.,LTD., we supply this compound in industrial purity grades suitable for large-scale manufacturing. Our standard product typically meets a purity of ≥98% by HPLC, with the exact specification confirmed on each batch-specific Certificate of Analysis (COA). For demanding applications, higher purities up to 99% can be provided upon request. The molecular formula is C23H29NO5, with a molecular weight of 399.48 g/mol. The compound appears as a white to off-white crystalline powder, and its identity is confirmed by 1H NMR and mass spectrometry.
When evaluating Fmoc-Thr(tBu)-OL for your synthesis route, it is essential to consider the impurity profile. Trace impurities, such as residual solvents or diastereomers, can affect coupling efficiency. Our manufacturing process is optimized to minimize these, ensuring consistent performance as an SPPS reagent. For a detailed comparison of typical purity grades, refer to the table below.
| Parameter | Standard Grade | High Purity Grade |
|---|---|---|
| Purity (HPLC) | ≥98% | ≥99% |
| Single Impurity | ≤1.0% | ≤0.5% |
| Water Content (KF) | ≤0.5% | ≤0.2% |
| Appearance | White to off-white powder | White crystalline powder |
These specifications are representative; please refer to the batch-specific COA for exact values. Our product is a direct Fmoc-Thr(tBu)-OL for peptide coupling, designed to meet the rigorous demands of modern peptide synthesis.
Batch-Specific COA Parameters and Analytical Methods
Every shipment of Fmoc-Thr(tBu)-OL from NINGBO INNO PHARMCHEM is accompanied by a comprehensive COA. This document details the analytical results for that specific batch, ensuring transparency and traceability. Key parameters include:
- Purity by HPLC: Determined using a C18 reverse-phase column with UV detection at 220 nm. The area normalization method is employed, and the chromatogram is provided for review.
- Optical Rotation: Measured at 20°C in chloroform (c=1). The specific rotation is typically around +15° to +18°, confirming the chiral integrity of the L-threonine derivative.
- Water Content: Karl Fischer titration is used to quantify moisture, which is critical for moisture-sensitive coupling reactions.
- Residual Solvents: Headspace GC-MS analysis ensures compliance with ICH Q3C guidelines for solvents like ethyl acetate or dichloromethane.
- Melting Point: Determined by differential scanning calorimetry (DSC), typically in the range of 95–100°C, though slight variations occur between batches.
We also provide additional testing upon request, such as heavy metals analysis or endotoxin levels for specialized applications. This rigorous quality control supports your manufacturing process and ensures that Fmoc-Thr(tBu)-OL performs reliably as a threonine derivative in your synthesis.
Industrial-Scale Bulk Packaging and Storage Conditions
For bulk procurement, NINGBO INNO PHARMCHEM offers Fmoc-Thr(tBu)-OL in a range of packaging options tailored to industrial needs. Standard packaging includes 1 kg, 5 kg, and 25 kg net weight per container. The product is packed in food-grade HDPE drums with double-layered LDPE liners, ensuring protection from moisture and contamination. For larger volumes, we can supply in 210L drums or intermediate bulk containers (IBCs) upon request. All packaging is labeled with the product name, CAS number (189337-28-8), batch number, and hazard pictograms in accordance with GHS standards.
Storage conditions are critical to maintaining product integrity. Fmoc-Thr(tBu)-OL should be stored at 2–8°C in a dry, well-ventilated area. Under these conditions, the product is stable for at least 12 months from the date of manufacture. Avoid exposure to strong bases or acids, as the Fmoc group is base-labile. When handling, use appropriate PPE including gloves and safety goggles. The product is classified as a combustible solid (storage class 11), so keep away from ignition sources. For logistics, we ensure secure transportation with temperature monitoring if required, though standard ambient shipping is acceptable for short durations.
Non-Standard Parameter: Viscosity Behavior at Sub-Zero Temperatures
While Fmoc-Thr(tBu)-OL is a solid at room temperature, its behavior in solution at low temperatures is a non-standard parameter that experienced chemists monitor. When dissolved in common solvents like DMF or dichloromethane, the solution viscosity can increase noticeably as temperatures approach -20°C. This is particularly relevant for automated SPPS instruments operating in cold rooms. In our field experience, a 0.5 M solution in DMF may exhibit a viscosity increase of 20–30% at -10°C compared to 20°C. This shift can affect flow rates and coupling kinetics if not accounted for. To mitigate this, we recommend pre-warming the solution to room temperature before use or adjusting the solvent ratio slightly. This hands-on knowledge helps avoid resin swelling inconsistencies and ensures smooth operation, as discussed in our related article on resolving resin swelling and steric hurdles with Fmoc-Thr(tBu)-OL.
Drop-in Replacement Strategy for Fmoc-Thr(tBu)-OH in Peptide Synthesis
Fmoc-Thr(tBu)-OL (CAS 189337-28-8) is structurally analogous to the widely used Fmoc-Thr(tBu)-OH (CAS 71989-35-0), with the key difference being the reduction of the carboxylic acid to a primary alcohol. This modification makes it a valuable building block for synthesizing peptide alcohols or C-terminally modified peptides. For many applications, Fmoc-Thr(tBu)-OL can serve as a seamless drop-in replacement, offering identical protecting group chemistry and comparable reactivity. The Fmoc group is removed under standard piperidine conditions, and the tBu ether is cleaved with TFA, just as with the acid counterpart.
From a procurement perspective, choosing Fmoc-Thr(tBu)-OL can yield cost efficiencies, especially at bulk scale. Our global manufacturing process is optimized for high yield, allowing us to offer competitive bulk pricing without compromising quality. Supply chain reliability is a cornerstone of our service; we maintain safety stock to buffer against demand fluctuations. When transitioning from Fmoc-Thr(tBu)-OH, minor adjustments to coupling protocols may be needed due to the alcohol functionality, but our technical team can provide guidance. For a deeper dive into the practical aspects, see our article on Fmoc-Thr(tBu)-OL and overcoming steric obstacles in peptide alcohol synthesis.
Frequently Asked Questions
What is the minimum order quantity (MOQ) for Fmoc-Thr(tBu)-OL?
Our standard MOQ is 1 kg for initial orders. For subsequent orders, we can accommodate smaller quantities upon request, subject to availability. Bulk pricing is available for orders of 25 kg or more.
Can you provide a sample for evaluation?
Yes, we offer free samples of up to 10 grams for qualified buyers. The sample will include a COA and MSDS. Shipping costs are typically borne by the requester.
What is the typical lead time for bulk orders?
For orders up to 25 kg, the lead time is usually 1–2 weeks after order confirmation. Larger quantities may require 3–4 weeks, depending on current production schedules. We provide regular updates throughout the process.
How do you ensure batch-to-batch consistency?
We employ a rigorous quality management system. Each batch is tested against established specifications, and a COA is issued. We also retain reference samples for at least two years to address any post-delivery inquiries.
Is Fmoc-Thr(tBu)-OL compatible with standard SPPS protocols?
Absolutely. The Fmoc and tBu protecting groups are fully compatible with standard Fmoc-SPPS chemistry. The alcohol functionality may require slight modifications in coupling or cleavage steps, but our technical support team can assist with protocol optimization.
Sourcing and Technical Support
At NINGBO INNO PHARMCHEM CO.,LTD., we are committed to being your reliable partner for Fmoc-Thr(tBu)-OL and other peptide synthesis building blocks. Our team combines deep chemical expertise with a customer-centric approach, ensuring you receive not only high-quality products but also the technical support needed to optimize your processes. Whether you are scaling up from lab to pilot or securing a long-term supply agreement, we are here to help. Partner with a verified manufacturer. Connect with our procurement specialists to lock in your supply agreements.