Intermediates

5-Azacytidine

  • CAS No.320-67-2
  • GradeIndustrial / Pharmaceutical
  • Availability● In Stock

High-purity 5-Azacytidine (CAS 320-67-2), a key nucleoside intermediate used in epigenetic research and pharmaceutical synthesis as a DNA demethylating agent.

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Product Technical Details

Product Overview

5-Azacytidine is a modified pyrimidine nucleoside widely employed in advanced pharmaceutical and biochemical research. As a structural analog of cytidine, it functions primarily as a potent inhibitor of DNA methyltransferase, thereby serving as a critical epigenetic modulator in cellular differentiation and gene expression studies. This compound is synthesized under stringent GMP-compliant conditions to ensure exceptional batch-to-batch consistency and suitability for use in early-stage drug development pipelines.

Specifications

Molecular FormulaC8H12N4O5
Molecular Weight244.21 g/mol
CAS Number320-67-2
AppearanceWhite or off-white crystalline powder
Assay (HPLC)≥98.0%
Loss on Drying≤1.0%
Residue on Ignition≤0.5%
Heavy Metals≤20 ppm
Melting Point226–232°C
Density2.08 g/cm³

Industrial Applications

As a specialized nucleoside intermediate, 5-Azacytidine is not intended for direct therapeutic use but serves as an essential building block in the synthesis of complex pharmaceutical APIs targeting oncology and hematological disorders. Its primary role lies in enabling epigenetic modulation during preclinical research, where it facilitates the study of DNA methylation pathways. Key applications include:

  • Supporting the development of novel epigenetic therapeutics through controlled demethylation mechanisms
  • Serving as a reference standard or reagent in molecular biology and cell culture assays
  • Acting as a precursor in multi-step organic syntheses requiring high-fidelity nucleoside scaffolds

Stored in a cool, dry, and well-ventilated environment, this intermediate maintains stability under recommended conditions. Packaging is typically provided in 25 kg fiber drums, with custom configurations available upon request to meet diverse R&D and pilot-scale manufacturing needs.